violetFluor™ 450 Anti-IL-2 Receptor alpha antibody [PC61.5] (ab242075)
![Flow Cytometry - violetFluor™ 450 Anti-IL-2 Receptor alpha antibody [PC61.5] (ab242075)](https://www.abcam.com/ps/products/242/ab242075/Images/ab242075-331042-anti-il-2-receptor-alpha-antibody-pc615-violetfluor-450-flow-cytometry.jpg "Flow Cytometry - violetFluor™ 450 Anti-IL-2 Receptor alpha antibody [PC61.5] (ab242075)")
Key features and details
- violetFluor™ 450 Rat monoclonal [PC61.5] to IL-2 Receptor alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: violetFluor™ 450
- Isotype: IgG1
Overview
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Product name
violetFluor™ 450 Anti-IL-2 Receptor alpha antibody [PC61.5]
See all IL-2 Receptor alpha primary antibodies -
Description
violetFluor™ 450 Rat monoclonal [PC61.5] to IL-2 Receptor alpha -
Host species
Rat -
Conjugation
violetFluor™ 450 -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: C57Bl/6 splenocytes.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
PC61.5 -
Isotype
IgG1 -
Light chain type
lambda -
Research areas
Associated products
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab242075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. 0.5 μg: The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. |
Target
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Function
Receptor for interleukin-2. -
Involvement in disease
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16184 Mouse
- SwissProt: P01590 Mouse
- Unigene: 915 Mouse
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Alternative names
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
Images
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Flow Cytometry - violetFluor™ 450 Anti-IL-2 Receptor alpha antibody [PC61.5] (ab242075)
Flow Cytometric analysis of C57Bl/6 bone marrow cells staining IL-2 Receptor alpha using 0.5 µg ab242075 (right panel) or 0.5 µg violet- Fluor™ 450 Rat IgG1 isotype control (left panel).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab242075 has not yet been referenced specifically in any publications.
