violetFluor™ 450 Anti-Interferon gamma antibody [XMG1.2] (ab253083)
![Flow Cytometry - violetFluor™ 450 Anti-Interferon gamma antibody [XMG1.2] (ab253083)](https://www.abcam.com/ps/products/253/ab253083/Images/ab253083-343656-antiinterferongammamonoclonalantibody-xmg12-flowcytometry-mouse.jpg "Flow Cytometry - violetFluor™ 450 Anti-Interferon gamma antibody [XMG1.2] (ab253083)")
Key features and details
- violetFluor™ 450 Rat monoclonal [XMG1.2] to Interferon gamma
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: violetFluor™ 450
- Isotype: IgG1
Overview
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Product name
violetFluor™ 450 Anti-Interferon gamma antibody [XMG1.2]
See all Interferon gamma primary antibodies -
Description
violetFluor™ 450 Rat monoclonal [XMG1.2] to Interferon gamma -
Host species
Rat -
Conjugation
violetFluor™ 450 -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: Mouse C57Bl/6 splenocytes.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
XMG1.2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab253083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. Use at 0.125 μg per reaction. |
Target
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Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 15978 Mouse
- SwissProt: P01580 Mouse
- Unigene: 240327 Mouse
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Alternative names
- IF 1 antibody
- IFG antibody
- IFI antibody
see all
Images
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Flow Cytometry - violetFluor™ 450 Anti-Interferon gamma antibody [XMG1.2] (ab253083)
Flow cytometric analysis of mouse C57Bl/6 splenocytes (stimulated with PMA and Ionomycin) stained for Interferon gamma using ab253083 at 0.125 μg per reaction (Right) or a rat IgG1 violetFluor™ 450 isotype control (Left).
Cells were first stained with an Anti-Mouse CD4 FITC conjugated antibody before intracellular staining for Interferon gamma with ab253083.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab253083 has not yet been referenced specifically in any publications.
