violetFluor™ 450 Anti-Ly6g antibody [1A8] (ab242074)

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Flow Cytometry - violetFluor™ 450 Anti-Ly6g antibody [1A8] (ab242074)

    Key features and details

    • violetFluor™ 450 Rat monoclonal [1A8] to Ly6g
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: violetFluor™ 450
    • Isotype: IgG2a

    Overview

    • Product name

      violetFluor™ 450 Anti-Ly6g antibody [1A8]
      See all Ly6g primary antibodies

    • Description

      violetFluor™ 450 Rat monoclonal [1A8] to Ly6g

    • Host species

      Rat

    • Conjugation

      violetFluor™ 450

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • Flow Cyt: Mouse C57Bl/6 bone marrow cells.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab242074 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    0.25 µg: The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. 

    Target

    • Relevance

      Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.

    • Cellular localization

      Attached to the membrane by a GPI-anchor.

    • Database links

      • Alternative names

        • Gr-1 antibody
        • Gr1 antibody
        • Ly-6G.1 antibody
        • Ly6G antibody
        • Lymphocyte antigen 6 complex locus G antibody
        • Lymphocyte antigen 6G antibody
        see all

      Images

      • Flow Cytometry - violetFluor™ 450 Anti-Ly6g antibody [1A8] (ab242074)
        Flow Cytometry - violetFluor™ 450 Anti-Ly6g antibody [1A8] (ab242074)

        Flow Cytometric analysis of C57Bl/6 bone marrow cells staining Ly6g using 0.25 µg ab242074 (right panel) or 0.25 µg violet- Fluor™ 450 Rat IgG2a isotype control (left panel).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab242074? Please let us know so that we can cite the reference in this datasheet.

      ab242074 has not yet been referenced specifically in any publications.

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