violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)

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Flow Cytometry - violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)

    Key features and details

    • violetFluor™ 450 Rat monoclonal [RB6-8C5] to Ly6g
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: violetFluor™ 450. Ex: 405nm, Em: 450nm
    • Isotype: IgG2b

    Conjugates logo Related conjugates and formulations

    APC Biotin CF405M FITC PE PE/Cy7® redFluor™ 710 Unconjugated

    Overview

    • Product name

      violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5]
      See all Ly6g primary antibodies

    • Description

      violetFluor™ 450 Rat monoclonal [RB6-8C5] to Ly6g

    • Host species

      Rat

    • Conjugation

      violetFluor™ 450. Ex: 405nm, Em: 450nm

    • Specificity

      In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • Flow cyt: Mouse C57Bl/6 bone marrow cells.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab253070 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use at an assay dependent concentration.

    Use at 0.06 μg per reaction.
    Notes
    Flow Cyt
    Use at an assay dependent concentration.

    Use at 0.06 μg per reaction.

    Target

    • Relevance

      Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.

    • Cellular localization

      Attached to the membrane by a GPI-anchor.

    • Database links

      • Alternative names

        • Gr-1 antibody
        • Gr1 antibody
        • Ly-6G.1 antibody
        • Ly6G antibody
        • Lymphocyte antigen 6 complex locus G antibody
        • Lymphocyte antigen 6G antibody
        see all

      Images

      • Flow Cytometry - violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)
        Flow Cytometry - violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)

        Flow cytometric analysis of mouse C57Bl/6 bone marrow cells stained for Ly6g using ab253070 at 0.06 μg per reaction (blue) or a rat IgG2b violetFluor™ 450 isotype control (black dashed line).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab253070? Please let us know so that we can cite the reference in this datasheet.

      ab253070 has not yet been referenced specifically in any publications.

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