violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)
![Flow Cytometry - violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)](https://www.abcam.com/ps/products/253/ab253070/Images/ab253070-343620-ab253070343620antily6gratmonoclonalantibodyrb68c5violetfluor450flowcytometrymouse.jpg "Flow Cytometry - violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)")
Key features and details
- violetFluor™ 450 Rat monoclonal [RB6-8C5] to Ly6g
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: violetFluor™ 450
- Isotype: IgG2b
Overview
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Product name
violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5]
See all Ly6g primary antibodies -
Description
violetFluor™ 450 Rat monoclonal [RB6-8C5] to Ly6g -
Host species
Rat -
Conjugation
violetFluor™ 450 -
Specificity
In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow cyt: Mouse C57Bl/6 bone marrow cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
RB6-8C5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- PerCP Anti-Ly6g antibody [RB6-8C5] (ab236728)
- redFluor™ 710 Anti-Ly6g antibody [RB6-8C5] (ab242065)
- PE Anti-Ly6g antibody [RB6-8C5] (ab24884)
- FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)
- APC Anti-Ly6g antibody [RB6-8C5] (ab25273)
- Anti-Ly6g antibody [RB6-8C5] (ab25377)
- PE/Cy7® Anti-Ly6g antibody [RB6-8C5] (ab25514)
- Biotin Anti-Ly6g antibody [RB6-8C5] (ab25680)
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab253070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. Use at 0.06 μg per reaction. |
Target
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Relevance
Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells. -
Cellular localization
Attached to the membrane by a GPI-anchor. -
Database links
- Entrez Gene: 546644 Mouse
- SwissProt: P35461 Mouse
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Alternative names
- Gr-1 antibody
- Gr1 antibody
- Ly-6G.1 antibody
see all
Images
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Flow Cytometry - violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)
Flow cytometric analysis of mouse C57Bl/6 bone marrow cells stained for Ly6g using ab253070 at 0.06 μg per reaction (blue) or a rat IgG2b violetFluor™ 450 isotype control (black dashed line).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab253070 has not yet been referenced specifically in any publications.
