violetFluor™ 450 Anti-TCR beta antibody [H57-597] (ab242073)

Submit a review Submit a question
Flow Cytometry - violetFluor™ 450 Anti-TCR beta antibody [H57-597] (ab242073)

    Key features and details

    • violetFluor™ 450 Armenian hamster monoclonal [H57-597] to TCR beta
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: violetFluor™ 450. Ex: 405nm, Em: 450nm
    • Isotype: IgG

    Overview

    • Product name

      violetFluor™ 450 Anti-TCR beta antibody [H57-597]
      See all TCR beta primary antibodies

    • Description

      violetFluor™ 450 Armenian hamster monoclonal [H57-597] to TCR beta

    • Host species

      Armenian hamster

    • Conjugation

      violetFluor™ 450. Ex: 405nm, Em: 450nm

    • Specificity

      The antibody does not cross-react with cells expressing the gamma-delta TCR.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • Flow Cyt: C57BI/6 splenocytes.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.

    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
    • Concentration information loading...

    • Purity

      Affinity purified

    • Purification notes

      ab242073 was purified from tissue culture supernatant via affinity chromatography.

    • Clonality

      Monoclonal

    • Clone number

      H57-597

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab242073 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use at an assay dependent concentration.

    0.25 µg: The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system.
    Notes
    Flow Cyt
    Use at an assay dependent concentration.

    0.25 µg: The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system.

    Target

    • Relevance

      TCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma and delta chains. The vast majority of circulating T cells (95%) express the alpha/beta heterodimer while roughly 2-5% express the gamma/delta heterodimer. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the TCR.

    • Cellular localization

      Plasma membrane.

    • Database links

      • Alternative names

        • MGC108929 antibody
        • MGC109620 antibody
        • MGC116333 antibody
        • MGC152215 antibody
        • MGC157076 antibody
        • MGC159579 antibody
        • MGC159580 antibody
        • RATTCB antibody
        • RATTCBC1 antibody
        • T cell antigen receptor, beta polypeptide, T cell receptor, beta cluster antibody
        • T cell receptor beta chain antibody
        • T cell receptor beta cluster antibody
        • T cell receptor beta locus antibody
        • T cell receptor beta/TCR VDJ BV1SI BJ1 antibody
        • T-cell antigen receptor, beta subunit antibody
        • T-cell receptor beta chain antibody
        • T-cell receptor beta chain V region CTL-L17 antibody
        • TCB antibody
        • TCBC1 antibody
        • TCRB antibody
        • Tcrb protein antibody
        • Tcrbb1-A antibody
        • TCRBCB1 antibody
        • TCRBCB2 antibody
        • TCRbeta antibody
        • Tib antibody
        • TRB antibody
        • TRB@ antibody
        see all

      Images

      • Flow Cytometry - violetFluor™ 450 Anti-TCR beta antibody [H57-597] (ab242073)
        Flow Cytometry - violetFluor™ 450 Anti-TCR beta antibody [H57-597] (ab242073)

        Flow cytometric analysis of C57BI/6 splenocytes labeling ab242073 (0.25 µg, solid line) compared to a IgG isotype control (Dashed line).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab242073? Please let us know so that we can cite the reference in this datasheet.

      ab242073 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab242073.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com