violetFluor™ 450 Anti-TCR beta antibody [H57-597] (ab242073)
![Flow Cytometry - violetFluor™ 450 Anti-TCR beta antibody [H57-597] (ab242073)](https://www.abcam.com/ps/products/242/ab242073/Images/ab242073-331056-474.jpg "Flow Cytometry - violetFluor™ 450 Anti-TCR beta antibody [H57-597] (ab242073)")
Key features and details
- violetFluor™ 450 Armenian hamster monoclonal [H57-597] to TCR beta
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: violetFluor™ 450
- Isotype: IgG
Overview
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Product name
violetFluor™ 450 Anti-TCR beta antibody [H57-597]
See all TCR beta primary antibodies -
Description
violetFluor™ 450 Armenian hamster monoclonal [H57-597] to TCR beta -
Host species
Armenian hamster -
Conjugation
violetFluor™ 450 -
Specificity
The antibody does not cross-react with cells expressing the gamma-delta TCR.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: C57BI/6 splenocytes.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
ab242073 was purified from tissue culture supernatant via affinity chromatography. -
Clonality
Monoclonal -
Clone number
H57-597 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- PE Anti-TCR beta antibody [H57-597] (ab236532)
- Biotin Anti-TCR beta antibody [H57-597] (ab236535)
- CF405M Anti-TCR beta antibody [H57-597] (ab236539)
- PerCP Anti-TCR beta antibody [H57-597] (ab236542)
- FITC Anti-TCR beta antibody [H57-597] (ab236545)
- APC Anti-TCR beta antibody [H57-597] (ab236572)
- FITC Anti-TCR alpha + TCR beta antibody [H57-597] (ab25010)
- Anti-TCR alpha + TCR beta antibody [H57-597] (ab25336)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab242073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. 0.25 µg: The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. |
Target
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Relevance
TCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma and delta chains. The vast majority of circulating T cells (95%) express the alpha/beta heterodimer while roughly 2-5% express the gamma/delta heterodimer. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the TCR. -
Cellular localization
Plasma membrane. -
Database links
- Entrez Gene: 21577 Mouse
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Alternative names
- MGC108929 antibody
- MGC109620 antibody
- MGC116333 antibody
see all
Images
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Flow Cytometry - violetFluor™ 450 Anti-TCR beta antibody [H57-597] (ab242073)
Flow cytometric analysis of C57BI/6 splenocytes labeling ab242073 (0.25 µg, solid line) compared to a IgG isotype control (Dashed line).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab242073 has not yet been referenced specifically in any publications.
