violetFluor™ 450 Anti-Thy1.2 antibody [30-H12] (ab242270)
![Flow Cytometry - violetFluor™ 450 Anti-Thy1.2 antibody [30-H12] (ab242270)](https://www.abcam.com/ps/products/242/ab242270/Images/ab242270-331264-2422701.jpg "Flow Cytometry - violetFluor™ 450 Anti-Thy1.2 antibody [30-H12] (ab242270)")
Key features and details
- violetFluor™ 450 Rat monoclonal [30-H12] to Thy1.2
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: violetFluor™ 450. Ex: 405nm, Em: 450nm
- Isotype: IgG2b
Overview
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Product name
violetFluor™ 450 Anti-Thy1.2 antibody [30-H12]
See all Thy1.2 primary antibodies -
Description
violetFluor™ 450 Rat monoclonal [30-H12] to Thy1.2 -
Host species
Rat -
Conjugation
violetFluor™ 450. Ex: 405nm, Em: 450nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Rat
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: C57Bl/6 splenocytes.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
30-H12 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab242270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
Use 0.25 µg. |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. Use 0.25 µg. |
Target
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Function
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 21838 Mouse
- SwissProt: P01831 Mouse
- Unigene: 3951 Mouse
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Alternative names
- CD90 antibody
- CD90 antigen antibody
- CD90.2 antibody
see all
Images
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Flow Cytometry - violetFluor™ 450 Anti-Thy1.2 antibody [30-H12] (ab242270)
Flow cytometric analysis of C57Bl/6 splenocytes staining CD90 / Thy1 using FITC Anti-Mouse CD3e and 0.25 µg ab242270 (right panel) or 0.25 µg violet- Fluor™ 450 Rat IgG2b isotype control (left panel).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab242270 has not yet been referenced specifically in any publications.
