The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This dilution applies to VIP coated directly at 2 µg/mL. For glutaraldehyde linked VIP ovalbumin (molar ratio 4:1) coated at 2 µg/mL, the dilution is 1/32,000.
VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. PHM and PHV also cause vasodilation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling VIP with ab30680 at a dilution of 1/100. Signal is strong in the nerves of the exocrine part of the pancreas.