Product nameAnti-VIP antibody
See all VIP primary antibodies
DescriptionRabbit polyclonal to VIP
Specificityab22736 reacts with Vasoactive Intestinal (poly)Peptide (VIP).
Tested applicationsSuitable for: IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Rat, Sheep, Dog, Human, Pig
Full length native protein (purified) (Pig) conjugated to KLH by a Glutaraldehyde linker.
- Human, rat and porcine colon and spinal cord.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: Whole serum
Our Abpromise guarantee covers the use of ab22736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/400 - 1/1600.
Notes: Dilute to working strength with PBS containing 0.1% w/v sodium azide.
Store diluted antibodies at 2-4°C(do not freeze) and use within 1 month.
using overnight incubation and indirect immunofluorescence procedure.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionVIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.
PHM and PHV also cause vasodilation.
Sequence similaritiesBelongs to the glucagon family.
- Information by UniProt
- Peptide histidine methioninamide 27 antibody
- Peptide histidine valine 42 antibody
- PHM27 antibody
ab22736 staining VIP in jejunum tissue section of pig by Immunohistochemistry (frozen sections). Tissue underwent fixation in paraformaldehyde and incubated with primary antibody, diluted 1/200 for 24 hours. A Texas Red® conjugated goat polyclonal to rabbit, dilution 1/400 was used as the secondary.
ab22736 staining VIP in sheep abomasum tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in paraformaldehyde without undergoing permeabilization and blocking steps. The primary antibody was diluted 1/1200 and incubated with sample for 24 hours. A FITC-conjugated goat polyclonal (IgG whole molecule) to rabbit IgG was diluted 1/400 and used as secondary.
This product has been referenced in:
- Niquille M et al. Neurogliaform cortical interneurons derive from cells in the preoptic area. Elife 7:N/A (2018). Read more (PubMed: 29557780) »
- Wasowicz K et al. Neuropeptides and lymphocyte populations in the porcine ileum and ileocecal lymph nodes during postnatal life. PLoS One 13:e0196458 (2018). Read more (PubMed: 29813072) »