Key features and details
- Rabbit polyclonal to VIP
- Suitable for: IHC-P
- Reacts with: Rat, Sheep, Dog, Human, Pig
- Isotype: IgG
Product nameAnti-VIP antibody
See all VIP primary antibodies
DescriptionRabbit polyclonal to VIP
Specificityab22736 reacts with Vasoactive Intestinal (poly)Peptide (VIP).
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Rat, Sheep, Dog, Human, Pig
Full length native protein (purified) (Pig) conjugated to KLH by a Glutaraldehyde linker.
- Human, rat and porcine colon and spinal cord.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab22736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/400 - 1/1600.
Notes: Dilute to working strength with PBS containing 0.1% w/v sodium azide.
Store diluted antibodies at 2-4°C(do not freeze) and use within 1 month.
using overnight incubation and indirect immunofluorescence procedure.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionVIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.
PHM and PHV also cause vasodilation.
Sequence similaritiesBelongs to the glucagon family.
- Information by UniProt
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ab22736 staining VIP in sheep abomasum tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in paraformaldehyde without undergoing permeabilization and blocking steps. The primary antibody was diluted 1/1200 and incubated with sample for 24 hours. A FITC-conjugated goat polyclonal (IgG whole molecule) to rabbit IgG was diluted 1/400 and used as secondary.
ab22736 has been referenced in 10 publications.
- Lucido CT et al. Innervation of cervical carcinoma is mediated by cancer-derived exosomes. Gynecol Oncol N/A:N/A (2019). PubMed: 31003747
- Xu X et al. Effect of Electroacupuncture on Hyperalgesia and Vasoactive Neurotransmitters in a Rat Model of Conscious Recurrent Migraine. Evid Based Complement Alternat Med 2019:9512875 (2019). PubMed: 31217804
- Madeo M et al. Cancer exosomes induce tumor innervation. Nat Commun 9:4284 (2018). PubMed: 30327461
- Niquille M et al. Neurogliaform cortical interneurons derive from cells in the preoptic area. Elife 7:N/A (2018). PubMed: 29557780
- Wasowicz K et al. Neuropeptides and lymphocyte populations in the porcine ileum and ileocecal lymph nodes during postnatal life. PLoS One 13:e0196458 (2018). PubMed: 29813072
- Chen QQ et al. Age-dependent alpha-synuclein accumulation and aggregation in the colon of a transgenic mouse model of Parkinson's disease. Transl Neurodegener 7:13 (2018). PubMed: 29988485
- Casalia ML et al. Persistent seizure control in epileptic mice transplanted with gamma-aminobutyric acid progenitors. Ann Neurol 82:530-542 (2017). PubMed: 28833459
- Zhong CB et al. Age-Dependent Alpha-Synuclein Accumulation and Phosphorylation in the Enteric Nervous System in a Transgenic Mouse Model of Parkinson's Disease. Neurosci Bull 33:483-492 (2017). PubMed: 28924920
- Nourinezhad J et al. Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig. Folia Histochem Cytobiol 55:221-229 (2017). PubMed: 29297566
- Hallett PJ et al. a-Synuclein overexpressing transgenic mice show internal organ pathology and autonomic deficits. Neurobiol Dis 47:258-67 (2012). PubMed: 22549133