Overview

  • Product name
  • Description
    Rabbit polyclonal to VIP
  • Host species
    Rabbit
  • Specificity
    This antibody reacts with a 3 kD vasointestinal peptide (VIP) localized in nerve fibers in the central and peripheral nervous system. The VIP producing tumors are usually neuroblastomas of endocrine tumors in the pancreas.
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length protein corresponding to Human VIP.

  • Positive control
    • IHC-P: Human nasal tissue.
  • General notes


    This antibody detects ganglion cells in both the superficial and deep plexus of the wall of the small bowel in human.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: 0.01% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    After solid-phase absorption this antibody is filtered through a 0.22 µm filter, vialed and lyophilized.
  • Primary antibody notes
    This antibody detects ganglion cells in both the superficial and deep plexus of the wall of the small bowel in human.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P 1/20.
ICC 1/50.
IHC-FoFr Use at an assay dependent concentration. PubMed: 29751824

Target

Images

  • Immunohistochemical analysis of human nasal tissue with allergic chronic rhinosinusitis, staining VIP with ab8556.

    Samples were blocked with blocking reagent for 10 minutes before incubating with primary antibody (1/50) at room temperature overnight. Staining was detected using DAB.

References

This product has been referenced in:
  • Puzan M  et al. Enteric Nervous System Regulation of Intestinal Stem Cell Differentiation and Epithelial Monolayer Function. Sci Rep 8:6313 (2018). Read more (PubMed: 29679034) »
  • Uemura N  et al. Inoculation of a-synuclein preformed fibrils into the mouse gastrointestinal tract induces Lewy body-like aggregates in the brainstem via the vagus nerve. Mol Neurodegener 13:21 (2018). IHC-FoFr ; Mouse . Read more (PubMed: 29751824) »

See all 4 Publications for this product

Customer reviews and Q&As

The stock solution of the Optiblot DTT Reducer (10X) ab119199 in 2M. This means that once diluted 10 times this produces a 200 mM solution. Therefore if you usually use 10 mM with your samples I would suggest diluting this 200 times. The higher concent...

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Thanks for keeping me updated.

I'm sorry to hear that the flow cytometry results were not so good. We haven't tested this antibody in flow so I don't have specific recommendations for a protocol, and it might requrie some optimization as you'...

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Thanks for your reply. After further discussion with the lab, I don't think I'll find out anything else about the IHC-Fr protocol, so I would recommend going ahead and trying the acetone fixation and some of the other alterations. If you do use up the ...

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Thanks again for your patience.

I've heard back from the lab, and they did not test ab8556 in-house on frozen tissue sections. This application was added to our datasheet based on feedback from an outside lab, and I haven't tracked down infor...

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Thank you for your call yesterday and for letting us know about the trouble with ab8556 in immunohistochemistry.

I apologize for the delay getting back to you,as I'm still waiting for more information from the lab regarding the suggested fixa...

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DISCOUNT CODE: xxxx Expiration date: xxxx I am very pleased to hear you would like to accept our offer and test ab8556 in Flow Cytometry. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an...

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Thank you very much for your interest in ab8556. To our knowledge, ab8556 has not been tested in Flow Cytometry. Therefore, I can offer a discount off a future purchase if you buy ab8556 now, test it in Flow Cytometry and submit feedback to us in t...

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Thank you for contacting me regarding this. It is important to have the code prior to ordering the first vial and to include that code as a reference in either your fax or email order, or to tell the customer service representative the code. They will ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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