Anti-VIP antibody - C-terminal (ab227850)
Key features and details
- Rabbit polyclonal to VIP - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-VIP antibody - C-terminal
See all VIP primary antibodies -
Description
Rabbit polyclonal to VIP - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Cow, Pig, Rhesus monkey
-
Immunogen
Synthetic peptide within Human VIP (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P01282 -
Positive control
- IHC-P: Mouse pancreas tissue. WB: SK-N-SH, PC-3 and SK-N-AS whole cell extracts.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 19 kDa.
|
|
| IHC-P |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 19 kDa. |
|
IHC-P
1/100 - 1/1000. |
Target
-
Function
VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.
PHM and PHV also cause vasodilation. -
Sequence similarities
Belongs to the glucagon family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 280956 Cow
- Entrez Gene: 7432 Human
- Entrez Gene: 22353 Mouse
- Entrez Gene: 100500718 Pig
- Entrez Gene: 117064 Rat
- Entrez Gene: 703566 Rhesus monkey
- Entrez Gene: 100145884 Sheep
- Omim: 192320 Human
see all -
Alternative names
- Peptide histidine methioninamide 27 antibody
- Peptide histidine valine 42 antibody
- PHM27 antibody
see all
Images
-
Western blot - Anti-VIP antibody - C-terminal (ab227850)Anti-VIP antibody - C-terminal (ab227850) at 1/1000 dilution + PC-3 (human prostate adenocarcinoma cell line) whole cell extract at 30 µg
Predicted band size: 19 kDa15% SDS-PAGE gel.
-
Western blot - Anti-VIP antibody - C-terminal (ab227850)All lanes : Anti-VIP antibody - C-terminal (ab227850) at 1/1000 dilution
Lane 1 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 2 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 19 kDa15% SDS-PAGE gel.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody - C-terminal (ab227850)Paraffin-embedded mouse pancreas tissue stained for VIP using ab227850 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab227850 has not yet been referenced specifically in any publications.