Anti-VIP antibody - C-terminal (ab227850)
Key features and details
- Rabbit polyclonal to VIP - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-VIP antibody - C-terminal
See all VIP primary antibodies -
Description
Rabbit polyclonal to VIP - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Cow, Pig, Rhesus monkey
-
Immunogen
Synthetic peptide within Human VIP (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P01282 -
Positive control
- IHC-P: Mouse pancreas tissue. WB: SK-N-SH, PC-3 and SK-N-AS whole cell extracts.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab227850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 19 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
-
Function
VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.
PHM and PHV also cause vasodilation. -
Sequence similarities
Belongs to the glucagon family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 280956 Cow
- Entrez Gene: 7432 Human
- Entrez Gene: 22353 Mouse
- Entrez Gene: 100500718 Pig
- Entrez Gene: 117064 Rat
- Entrez Gene: 703566 Rhesus monkey
- Entrez Gene: 100145884 Sheep
- Omim: 192320 Human
see all -
Alternative names
- Peptide histidine methioninamide 27 antibody
- Peptide histidine valine 42 antibody
- PHM27 antibody
see all
Images
-
Western blot - Anti-VIP antibody - C-terminal (ab227850)Anti-VIP antibody - C-terminal (ab227850) at 1/1000 dilution + PC-3 (human prostate adenocarcinoma cell line) whole cell extract at 30 µg
Predicted band size: 19 kDa15% SDS-PAGE gel.
-
Western blot - Anti-VIP antibody - C-terminal (ab227850)All lanes : Anti-VIP antibody - C-terminal (ab227850) at 1/1000 dilution
Lane 1 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 2 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 19 kDa15% SDS-PAGE gel.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody - C-terminal (ab227850)Paraffin-embedded mouse pancreas tissue stained for VIP using ab227850 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227850 has not yet been referenced specifically in any publications.
