Anti-VIP antibody - C-terminal (ab227850)
Rabbit polyclonal VIP antibody. Validated in WB, IHC and tested in Mouse, Human. Immunogen corresponding to synthetic peptide.
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-VIP antibody - C-terminal
See all VIP primary antibodies -
Description
Rabbit polyclonal to VIP - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Cow, Pig, Rhesus monkey
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Immunogen
Synthetic peptide within Human VIP (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P01282 -
Positive control
- IHC-P: Mouse pancreas tissue. WB: SK-N-SH, PC-3 and SK-N-AS whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 19 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.
PHM and PHV also cause vasodilation. -
Sequence similarities
Belongs to the glucagon family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 280956 Cow
- Entrez Gene: 7432 Human
- Entrez Gene: 22353 Mouse
- Entrez Gene: 100500718 Pig
- Entrez Gene: 117064 Rat
- Entrez Gene: 703566 Rhesus monkey
- Entrez Gene: 100145884 Sheep
- Omim: 192320 Human
see all -
Alternative names
- Peptide histidine methioninamide 27 antibody
- Peptide histidine valine 42 antibody
- PHM27 antibody
see all
Images
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Western blot - Anti-VIP antibody - C-terminal (ab227850)Anti-VIP antibody - C-terminal (ab227850) at 1/1000 dilution + PC-3 (human prostate adenocarcinoma cell line) whole cell extract at 30 µg
Predicted band size: 19 kDa15% SDS-PAGE gel.
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Western blot - Anti-VIP antibody - C-terminal (ab227850)All lanes : Anti-VIP antibody - C-terminal (ab227850) at 1/1000 dilution
Lane 1 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 2 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 19 kDa15% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody - C-terminal (ab227850)Paraffin-embedded mouse pancreas tissue stained for VIP using ab227850 at 1/500 dilution in immunohistochemical analysis.
References
ab227850 has not yet been referenced specifically in any publications.
