Anti-VIP2 antibody (ab154046)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-VIP2 antibody
See all VIP2 primary antibodies -
Description
Rabbit polyclonal to VIP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to Human VIP2 aa 5-341.
Database link: O43314 -
Positive control
- Raji and K562 whole cell lysates; Human PC13 xenograft tissue.
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General notes
Previously labelled as PPIP5K2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 140 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
Bifunctional inositol kinase that catalyzes the formation of diphosphoinositol pentakisphosphate (InsP7 or PP-InsP5) and bi-diphosphoinositol tetrakisphosphate (InsP8 or PP2-InsP4). Converts inositolitol hexakisphosphate (InsP6) to InsP7. Also able to convert InsP7 to InsP8. Probably specifically mediates the formation of 4PP-InsP5 and 6PP-InsP5 InsP7 isomers but not of 5PP-IP5 InsP7 isomer. -
Sequence similarities
Belongs to the histidine acid phosphatase family. VIP1 subfamily. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 780855 Cow
- Entrez Gene: 23262 Human
- Entrez Gene: 227399 Mouse
- Entrez Gene: 495012 Xenopus laevis
- Entrez Gene: 100135086 Xenopus tropicalis
- Omim: 611648 Human
- SwissProt: O43314 Human
- SwissProt: Q6ZQB6 Mouse
see all -
Alternative names
- Diphosphoinositol pentakisphosphate kinase 2 antibody
- HISPPD1 antibody
- Histidine acid phosphatase domain containing 1 antibody
see all
Images
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Western blot - Anti-VIP2 antibody (ab154046)All lanes : Anti-VIP2 antibody (ab154046) at 1/3000 dilution
Lane 1 : Raji whole cell lysate
Lane 2 : K562 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 140 kDa
5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP2 antibody (ab154046)Immunohistochemical analysis of paraffin-embedded Human PC13 xenograft tissue, labeling VIP2 with ab154046 at 1/500 dilution.
References
This product has been referenced in:
- Gu C et al. Inositol Pyrophosphate Profiling of Two HCT116 Cell Lines Uncovers Variation in InsP8 Levels. PLoS One 11:e0165286 (2016). WB ; Human . Read more (PubMed: 27788189) »
