Anti-VIP2 antibody (ab154046)
Key features and details
- Rabbit polyclonal to VIP2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VIP2 antibody
See all VIP2 primary antibodies -
Description
Rabbit polyclonal to VIP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to Human VIP2 aa 5-341.
Database link: O43314 -
Positive control
- Raji and K562 whole cell lysates; Human PC13 xenograft tissue.
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General notes
Previously labelled as PPIP5K2.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 140 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
Bifunctional inositol kinase that catalyzes the formation of diphosphoinositol pentakisphosphate (InsP7 or PP-InsP5) and bi-diphosphoinositol tetrakisphosphate (InsP8 or PP2-InsP4). Converts inositolitol hexakisphosphate (InsP6) to InsP7. Also able to convert InsP7 to InsP8. Probably specifically mediates the formation of 4PP-InsP5 and 6PP-InsP5 InsP7 isomers but not of 5PP-IP5 InsP7 isomer. -
Sequence similarities
Belongs to the histidine acid phosphatase family. VIP1 subfamily. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 780855 Cow
- Entrez Gene: 23262 Human
- Entrez Gene: 227399 Mouse
- Entrez Gene: 495012 Xenopus laevis
- Entrez Gene: 100135086 Xenopus tropicalis
- Omim: 611648 Human
- SwissProt: O43314 Human
- SwissProt: Q6ZQB6 Mouse
see all -
Alternative names
- Diphosphoinositol pentakisphosphate kinase 2 antibody
- HISPPD1 antibody
- Histidine acid phosphatase domain containing 1 antibody
see all
Images
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Western blot - Anti-VIP2 antibody (ab154046)All lanes : Anti-VIP2 antibody (ab154046) at 1/3000 dilution
Lane 1 : Raji whole cell lysate
Lane 2 : K562 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 140 kDa
5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP2 antibody (ab154046)Immunohistochemical analysis of paraffin-embedded Human PC13 xenograft tissue, labeling VIP2 with ab154046 at 1/500 dilution.
Protocols
References (1)
ab154046 has been referenced in 1 publication.
- Gu C et al. Inositol Pyrophosphate Profiling of Two HCT116 Cell Lines Uncovers Variation in InsP8 Levels. PLoS One 11:e0165286 (2016). WB ; Human . PubMed: 27788189
