Overview

  • Product name

  • Description

    Rabbit polyclonal to VIP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Guinea pig, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide within Human VIP2 aa 925-975. The exact sequence is proprietary.
    Database link: O43314

  • Positive control

    • K562, HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 whole cell lysates.
  • General notes

    Previously labelled as PPIP5K2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab183527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 140 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Bifunctional inositol kinase that catalyzes the formation of diphosphoinositol pentakisphosphate (InsP7 or PP-InsP5) and bi-diphosphoinositol tetrakisphosphate (InsP8 or PP2-InsP4). Converts inositolitol hexakisphosphate (InsP6) to InsP7. Also able to convert InsP7 to InsP8. Probably specifically mediates the formation of 4PP-InsP5 and 6PP-InsP5 InsP7 isomers but not of 5PP-IP5 InsP7 isomer.
  • Sequence similarities

    Belongs to the histidine acid phosphatase family. VIP1 subfamily.
  • Cellular localization

    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • Diphosphoinositol pentakisphosphate kinase 2 antibody
    • HISPPD1 antibody
    • Histidine acid phosphatase domain containing 1 antibody
    • Histidine acid phosphatase domain containing protein 1 antibody
    • Histidine acid phosphatase domain-containing protein 1 antibody
    • hsVIP2 antibody
    • Inositol heptaphosphate kinase 2 antibody
    • Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2 antibody
    • InsP6 and PP IP5 kinase 2 antibody
    • InsP6 and PP-IP5 kinase 2 antibody
    • IP7K2 antibody
    • PP IP5 kinase 2 antibody
    • Ppip5k2 antibody
    • VIP1 homolog 2 antibody
    • VIP2 antibody
    • VIP2_HUMAN antibody
    see all

Images

  • Detection of VIP2 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab183527 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab183527 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

  • All lanes : Anti-VIP2 antibody (ab183527) at 0.4 µg/ml

    Lane 1 : K562 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : 293T whole cell lysate
    Lane 4 : Jurkat whole cell lysate
    Lane 5 : Mouse TCMK-1 whole cell lysate
    Lane 6 : Mouse NIH-3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 140 kDa


    Exposure time: 10 seconds

References

ab183527 has not yet been referenced specifically in any publications.

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