Anti-VIP36 antibody (ab224227)
Key features and details
- Rabbit polyclonal to VIP36
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VIP36 antibody
See all VIP36 primary antibodies -
Description
Rabbit polyclonal to VIP36 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Dog -
Immunogen
Recombinant fragment corresponding to Human VIP36 aa 1-200.
Database link: Q12907 -
Positive control
- WB: VIP36 overexpression HEK-293T lysate. IHC-P: Human testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 40 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 40 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plays a role as an intracellular lectin in the early secretory pathway. Interacts with N-acetyl-D-galactosamine and high-mannose type glycans and may also bind to O-linked glycans. Involved in the transport and sorting of glycoproteins carrying high mannose-type glycans. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 L-type lectin-like domain. -
Cellular localization
Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 403938 Dog
- Entrez Gene: 10960 Human
- Entrez Gene: 66890 Mouse
- Omim: 609551 Human
- SwissProt: P49256 Dog
- SwissProt: Q12907 Human
- SwissProt: Q9DBH5 Mouse
- Unigene: 75864 Human
see all -
Alternative names
- C5orf8 antibody
- Glycoprotein GP36b antibody
- GP36B antibody
see all
Images
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Paraffin-embedded human skeletal muscle stained for VIP36 using ab224227 at 1/50 dilution in immunohistochemical analysis. Shows no positivity in myocytes as expected.
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Paraffin-embedded human testis stained for VIP36 using ab224227 at 1/50 dilution in immunohistochemical analysis. Shows moderate cytoplasmic positivity in cells of the seminiferous duct.
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Paraffin-embedded human lungs stained for VIP36 using ab224227 at 1/50 dilution in immunohistochemical analysis. Shows weak to moderate cytoplasmic positivity in macrophages.
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All lanes : Anti-VIP36 antibody (ab224227) at 1/100 dilution
Lane 1 : Vector only transfected HEK293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) overexpressing VIP36 (co-expressed with a C terminal myc-DDK tag), cell lysate
Developed using the ECL technique.
Predicted band size: 40 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224227 has not yet been referenced specifically in any publications.