Key features and details
- Rabbit polyclonal to VIP36
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-VIP36 antibody
See all VIP36 primary antibodies
DescriptionRabbit polyclonal to VIP36
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Dog
- WB: VIP36 overexpression HEK-293T lysate. IHC-P: Human testis tissue.
Previously labelled as LMAN2.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 40 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlays a role as an intracellular lectin in the early secretory pathway. Interacts with N-acetyl-D-galactosamine and high-mannose type glycans and may also bind to O-linked glycans. Involved in the transport and sorting of glycoproteins carrying high mannose-type glycans.
Sequence similaritiesContains 1 L-type lectin-like domain.
Cellular localizationEndoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- C5orf8 antibody
- Glycoprotein GP36b antibody
- GP36B antibody
Paraffin-embedded human skeletal muscle stained for VIP36 using ab224227 at 1/50 dilution in immunohistochemical analysis. Shows no positivity in myocytes as expected.
Paraffin-embedded human testis stained for VIP36 using ab224227 at 1/50 dilution in immunohistochemical analysis. Shows moderate cytoplasmic positivity in cells of the seminiferous duct.
Paraffin-embedded human lungs stained for VIP36 using ab224227 at 1/50 dilution in immunohistochemical analysis. Shows weak to moderate cytoplasmic positivity in macrophages.
All lanes : Anti-VIP36 antibody (ab224227) at 1/100 dilution
Lane 1 : Vector only transfected HEK293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) overexpressing VIP36 (co-expressed with a C terminal myc-DDK tag), cell lysate
Developed using the ECL technique.
Predicted band size: 40 kDa
ab224227 has not yet been referenced specifically in any publications.