Key features and details
- Rabbit polyclonal to Viperin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Viperin antibody
See all Viperin primary antibodies
DescriptionRabbit polyclonal to Viperin
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Horse, Dog, Chimpanzee, Orangutan
Synthetic peptide corresponding to Human Viperin aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following Lysates: Mouse Spinal Cord Tissue, SK N SH Whole Cell, Rat Spinal Cord Tissue, Human Brain Tissue, Mouse Brain Tissue It also gave a positive result in U87-MG cell line
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab73864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
FunctionInvolved in antiviral defense. May impair virus budding by disrupting lipid rafts at the plasma membrane, a feature which is essential for the budding process of many viruses. Acts through binding with and inactivating FPPS, an enzyme involved in synthesis of cholesterol, farnesylated and geranylated proteins, ubiquinones dolichol and heme. Plays a major role in the cell antiviral state induced by type I and type II interferon. Displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses, but not vesiculovirus.
Sequence similaritiesBelongs to the RSAD2 family.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus. Probably associates with the cytosolic side of the endoplasmic reticulum. Infection with human cytomegalovirus (HCMV) causes relocation to the Golgi apparatus and to cytoplasmic vacuoles which also contain HCMV proteins glycoprotein B and pp28.
- Information by UniProt
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All lanes : Anti-Viperin antibody (ab73864) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 3 : Spinal Cord (Rat) Tissue Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lane 5 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds
ICC/IF image of ab78364 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73864, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab73864 has been referenced in 4 publications.
- Upadhyay AS et al. Cellular requirements for iron-sulfur cluster insertion into the antiviral radical SAM protein viperin. J Biol Chem 292:13879-13889 (2017). PubMed: 28615450
- Mangeat B et al. Influenza virus partially counteracts restriction imposed by tetherin/BST-2. J Biol Chem 287:22015-29 (2012). WB . PubMed: 22493439
- Kroeker AL et al. Response of primary human airway epithelial cells to influenza infection: a quantitative proteomic study. J Proteome Res 11:4132-46 (2012). WB . PubMed: 22694362
- Mboko WP et al. Coordinate regulation of DNA damage and type I interferon responses imposes an antiviral state that attenuates mouse gammaherpesvirus type 68 replication in primary macrophages. J Virol 86:6899-912 (2012). WB . PubMed: 22496235