Overview

  • Product name

  • Description

    Rabbit polyclonal to Viperin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Dog, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Viperin aa 250-350 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab73863)

  • Positive control

    • This antibody gave a positive signal in the following Lysates: Mouse Spinal Cord Tissue, SK N SH Whole Cell, Rat Spinal Cord Tissue, Human Brain Tissue, Mouse Brain Tissue It also gave a positive result in U87-MG cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab73864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • Function

    Involved in antiviral defense. May impair virus budding by disrupting lipid rafts at the plasma membrane, a feature which is essential for the budding process of many viruses. Acts through binding with and inactivating FPPS, an enzyme involved in synthesis of cholesterol, farnesylated and geranylated proteins, ubiquinones dolichol and heme. Plays a major role in the cell antiviral state induced by type I and type II interferon. Displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses, but not vesiculovirus.
  • Sequence similarities

    Belongs to the RSAD2 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus. Probably associates with the cytosolic side of the endoplasmic reticulum. Infection with human cytomegalovirus (HCMV) causes relocation to the Golgi apparatus and to cytoplasmic vacuoles which also contain HCMV proteins glycoprotein B and pp28.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2510004L01Rik antibody
    • cig 33 antibody
    • CIG 5 antibody
    • cig-33 antibody
    • CIG-5 antibody
    • CIG33 antibody
    • CIG5 antibody
    • Cytomegalovirus induced gene 5 protein antibody
    • Cytomegalovirus-induced gene 5 protein antibody
    • endoplasmic reticulum-associated antibody
    • interferon-inducible antibody
    • Radical S adenosyl methionine domain containing 2 antibody
    • Radical S-adenosyl methionine domain-containing protein 2 antibody
    • RSAD 2 antibody
    • Rsad2 antibody
    • RSAD2_HUMAN antibody
    • RSDA-2 antibody
    • VIG 1 antibody
    • vig1 antibody
    • Viperin antibody
    • Virus inhibitory protein antibody
    • virus inhibitory protein endoplasmic reticulum associated interferon inducible antibody
    see all

Images

  • All lanes : Anti-Viperin antibody (ab73864) at 1 µg/ml

    Lane 1 : Spinal Cord (Mouse) Tissue Lysate
    Lane 2 : SK N SH (Human neuroblastoma) Whole Cell Lysate
    Lane 3 : Spinal Cord (Rat) Tissue Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)
    Lane 5 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 30 seconds
  • ICC/IF image of ab78364 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73864, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Upadhyay AS  et al. Cellular requirements for iron-sulfur cluster insertion into the antiviral radical SAM protein viperin. J Biol Chem 292:13879-13889 (2017). Read more (PubMed: 28615450) »
  • Mangeat B  et al. Influenza virus partially counteracts restriction imposed by tetherin/BST-2. J Biol Chem 287:22015-29 (2012). WB . Read more (PubMed: 22493439) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

DISCOUNT CODE: XXXX
Expiration date: 03-08-2013
Value: €380

I am very pleased to hear you would like to accept our offer and test ab73864 in Porcine. This code will give you €380 off your next order before the expiration date. To redeem this offer, please submit an Abreview for Porcine and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Answer

Thank you for contacting us.
The immunogen is 84% homologous to Porcine sequence. Though this antibody has not been tested but it is more likely to work.
In case you are interested in trying this antibody then I would encourage you to participate in our 100% Abtrial discount programme - a free product can be claimed in exchange of review for this antibody. Please contact me for code if you accept our offer.
More details about the offer can be found at the following link;
https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Vielen Dank für Ihre Anfrage.

Es freut mich zu hören, dass ab73864 im Western blot gut funktioniert.

Wir haben diesen Antikörper bisher noch nicht in der Immunhistochemie (IHC) getestet, und wissen daher leider nicht, ob bzw. wie er funktionieren könnte.

Wenn wir Antikörper für die IHC testen, probieren wir in der Regel zwei verschiedene Konzentrationen (1 und 5 µg/ml) und jeweils zwei unterschiedliche Probenpräparationen aus. Letztere sind zum Beispiel entweder Paraformaldehyd- (PFA) oder Aceton-fixierte Gefrierschnitte, oder beispielsweise mit Zitratpuffer pH6 oder Tris/EDTA-Puffer pH9 demaskierte Paraffinschnitte. Je nach Ergebnis können die Konzentration und die Versuchsbedingungen dann noch weiter optimiert werden. Für weitere Details habe ich Ihnen unsere Protokolle angehangen, die Sie auch auf unserer Webseite finden (https://www.abcam.com/index.html?pageconfig=popular_protocols).

Falls Sie mit diesem Antikörper gute Ergebnisse in der IHC erzielen, würden wir uns über eine Rückmeldung mittels eines Abreviews freuen. Das Abreview-System erlaubt es unseren Kunden, Rezensionen unserer Produkte zu veröffentlichen und dafür Punkte zu erhalten. Diese “Abpoints” können gegen Rabatte beim Kauf weiterer Produkte oder Geschenkgutscheine wie z.B. Amazon.com-Gutscheine eingetauscht werden. Unter der folgenden URL können Sie mehr über unser Abreview-System zu erfahren: https://www.abcam.com/abreviews

Und noch ein Tipp: Falls Sie den Antikörperwieder neu bestellen müssen, geben Sie uns vorher bescheid. Dann können wir Sie nämlich in unser Testprogramm aufnehmen, bei dem Sie einen kostenlosen Antikörper bekommen, wenn Sie uns Ihre Testergebnisse mitteilen. (Leider können wir den entsprechenden Rabattcode nicht nachträglich ausstellen.) Weitere Informationen finden Sie unter: https://www.abcam.com/collaborationdiscount

Ich hoffe, diese Informationen helfen Ihnen weiter, und wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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