Anti-Viperin antibody [MaP.VIP] (ab107359)
Key features and details
- Mouse monoclonal [MaP.VIP] to Viperin
- Suitable for: ICC/IF, IP, Flow Cyt, WB
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Viperin antibody [MaP.VIP]
See all Viperin primary antibodies -
Description
Mouse monoclonal [MaP.VIP] to Viperin -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IP, Flow Cyt, WBmore details
Unsuitable for: IHC-Fr -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment corresponding to amino acids 92-362 of Mouse Viperin
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MaP.VIP -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab107359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
The typical starting working dilution is 1:50. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
The typical starting working dilution is 1:50. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. The typical starting working dilution is 1:50. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa. The typical starting working dilution is 1:50. |
Target
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Function
Involved in antiviral defense. May impair virus budding by disrupting lipid rafts at the plasma membrane, a feature which is essential for the budding process of many viruses. Acts through binding with and inactivating FPPS, an enzyme involved in synthesis of cholesterol, farnesylated and geranylated proteins, ubiquinones dolichol and heme. Plays a major role in the cell antiviral state induced by type I and type II interferon. Displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses, but not vesiculovirus. -
Sequence similarities
Belongs to the RSAD2 family. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus. Probably associates with the cytosolic side of the endoplasmic reticulum. Infection with human cytomegalovirus (HCMV) causes relocation to the Golgi apparatus and to cytoplasmic vacuoles which also contain HCMV proteins glycoprotein B and pp28. - Information by UniProt
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Database links
- Entrez Gene: 91543 Human
- Entrez Gene: 58185 Mouse
- Entrez Gene: 65190 Rat
- Omim: 607810 Human
- SwissProt: Q8WXG1 Human
- SwissProt: Q8CBB9 Mouse
- SwissProt: O70600 Rat
- Unigene: 17518 Human
see all -
Alternative names
- 2510004L01Rik antibody
- cig 33 antibody
- CIG 5 antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (7)
ab107359 has been referenced in 7 publications.
- Yuan Y et al. High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction. EMBO Rep 23:e53466 (2022). PubMed: 34779558
- Bouvet M et al. Multiple Viral microRNAs Regulate Interferon Release and Signaling Early during Infection with Epstein-Barr Virus. mBio 12:N/A (2021). PubMed: 33785626
- Sarkar R et al. Viperin, an IFN-Stimulated Protein, Delays Rotavirus Release by Inhibiting Non-Structural Protein 4 (NSP4)-Induced Intrinsic Apoptosis. Viruses 13:N/A (2021). PubMed: 34372530
- Lindqvist R et al. Cell-type- and region-specific restriction of neurotropic flavivirus infection by viperin. J Neuroinflammation 15:80 (2018). WB ; Mouse . PubMed: 29544502
- Upadhyay AS et al. Cellular requirements for iron-sulfur cluster insertion into the antiviral radical SAM protein viperin. J Biol Chem 292:13879-13889 (2017). PubMed: 28615450
- Van der Hoek KH et al. Viperin is an important host restriction factor in control of Zika virus infection. Sci Rep 7:4475 (2017). PubMed: 28667332
- Tang HB et al. Viperin inhibits rabies virus replication via reduced cholesterol and sphingomyelin and is regulated upstream by TLR4. Sci Rep 6:30529 (2016). WB ; Mouse . PubMed: 27456665