Key features and details
- Rabbit polyclonal to Viral Protein U
- Suitable for: ELISA, WB, ICC/IF, IP
- Isotype: IgG
Product nameAnti-Viral Protein U antibody
DescriptionRabbit polyclonal to Viral Protein U
Tested applicationsSuitable for: ELISA, WB, ICC/IF, IPmore details
Species reactivityReacts with: Other species
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Proprietary antibody stabilization buffer.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab81532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Positive for Cells transfected with Vpu|
RelevanceRetroviruses have several characteristic structural and catalytic proteins. One such auxiliary protein is a viral protein U (Vpu) which enhances virion release from human cells and is also involved in the degradation of CD4, the cellular surface receptor of HIV-1. The Vpu has no homolog in less pathogenic HIV-2 virus. Vpu is an 81 amino acid class I membrane integral protein that is unique to human and simian immunodeficiency virus isolated from Chimpanzee and a few other monkey species. The 16kDa protein Vpu protein consists of an N-terminal hydrophobic membrane anchor of 27 amino acids and a charged C-terminal hydrophilic domain of 54 amino acids that extents to the cytoplasm. The cytoplasmic domain has a conserved dual serine phosphorylation site (S52 GXX and S56 motif) that is phosphorylated by casein kinase II. Vpu is involved in viral replication and degradation of its cellular receptor CD4 and enhancement of viral particle release from macrophages and primary lymphocytes.
Cellular localizationMembrane; Single-pass type I membrane protein.
- U ORF protein antibody
- Vpu antibody
ab81532 has been referenced in 1 publication.
- Swann J et al. Cytosolic sulfotransferase 1A1 regulates HIV-1 minus-strand DNA elongation in primary human monocyte-derived macrophages. Virol J 13:30 (2016). PubMed: 26906565