Overview

  • Product name

    Anti-Visfatin [14A5] antibody
    See all Visfatin primary antibodies
  • Description

    Rat monoclonal [14A5] to Visfatin
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Visfatin. 6xHis-tagged mouse
    Sequence:

    MAAAVVDPQQSVVMRV-C


    Database link: Q99KQ4

  • Positive control

    • Mouse spleen frozen sections; fetal mouse skin dendritic cell line (FSDC).

Properties

Applications

Our Abpromise guarantee covers the use of ab192738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 55 kDa.
ELISA 1/5000 - 1/20000.
IHC-Fr 1/100 - 1/400.

Target

  • Function

    Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
  • Tissue specificity

    Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
  • Pathway

    Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
  • Sequence similarities

    Belongs to the NAPRTase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1110035O14Rik antibody
    • AI314458 antibody
    • AI480535 antibody
    • DKFZP666B131 antibody
    • EC 2.4.2.12 antibody
    • MGC117256 antibody
    • NAmPRTase antibody
    • Nampt antibody
    • NAMPT_HUMAN antibody
    • Nicotinamide phosphoribosyltransferase antibody
    • PBEF 1 antibody
    • PBEF antibody
    • PBEF1 antibody
    • Pre B cell colony enhancing factor 1 antibody
    • Pre B cell colony enhancing factor antibody
    • Pre B cell enhancing factor antibody
    • Pre-B cell-enhancing factor antibody
    • Pre-B-cell colony-enhancing factor 1 antibody
    • VF antibody
    • Visfatin antibody
    see all

Images

  • Immunohistochemical analysis of mouse spleen frozen sections labeling Visfatin with ab192738 at 10 μg/ml (Green) in distinct spleen cell populations (CD11c positive and negative). Secondary Ab: anti-Rat-Alexa488, anti- CD11c-Alexa568.

  • Immunohistochemical analysis of frozen mouse spleen tissue labeling Visfatin with ab192738 at 10 μg/ml. A Biotinylated anti-Rat antibody was used for secondary detection.

  • Anti-Visfatin [14A5] antibody (ab192738) at 0.1 µg/ml + fetal mouse skin dendritic cell line (FSDC) extract

    Secondary
    anti rat HPRT

    Predicted band size: 55 kDa

References

ab192738 has not yet been referenced specifically in any publications.

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