Overview

  • Product name

  • Description

    Rabbit polyclonal to Visfatin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow, Pig, Xenopus laevis
  • Immunogen

    Recombinant fragment within Human Visfatin (internal sequence). The exact sequence is proprietary.
    Database link: P43490

  • Positive control

    • WB: A431, Raji, Jurkat, K562, NCI-H929, A549, HCT 116 and MCF7 whole cell lysates. IHC-P: Rat liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.

Target

  • Function

    Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
  • Tissue specificity

    Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
  • Pathway

    Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
  • Sequence similarities

    Belongs to the NAPRTase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1110035O14Rik antibody
    • AI314458 antibody
    • AI480535 antibody
    • DKFZP666B131 antibody
    • EC 2.4.2.12 antibody
    • MGC117256 antibody
    • NAmPRTase antibody
    • Nampt antibody
    • NAMPT_HUMAN antibody
    • Nicotinamide phosphoribosyltransferase antibody
    • PBEF 1 antibody
    • PBEF antibody
    • PBEF1 antibody
    • Pre B cell colony enhancing factor 1 antibody
    • Pre B cell colony enhancing factor antibody
    • Pre B cell enhancing factor antibody
    • Pre-B cell-enhancing factor antibody
    • Pre-B-cell colony-enhancing factor 1 antibody
    • VF antibody
    • Visfatin antibody
    see all

Images

  • Paraffin-embedded rat liver tissue stained for Visfatin using ab227474 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-Visfatin antibody (ab227474) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 56 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-Visfatin antibody (ab227474) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 4 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 56 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-Visfatin antibody (ab227474) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 56 kDa



    7.5% SDS-PAGE gel.

References

ab227474 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227474.
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