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Cardiovascular Hypoxia Hypoxia-Regulated
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Anti-Visfatin antibody (ab24149)

  • Datasheet
Reviews (1) Submit a question References (4)

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Western blot - Anti-Visfatin antibody (ab24149)

    Key features and details

    • Rabbit polyclonal to Visfatin
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant human Visfatin protein (ab198090)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Visfatin antibody
      See all Visfatin primary antibodies
    • Description

      Rabbit polyclonal to Visfatin
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide:

      SFDEIRKNAQ LNIELEAAHH

      , corresponding to C terminal amino acids 472-491 of Human PBEF.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Purchase matching WB positive control:Recombinant Human Visfatin protein

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Hypoxia
      • Hypoxia-Regulated
      • Cardiovascular
      • Blood
      • Serum Proteins
      • Immunology
      • Adaptive Immunity
      • B Cells
      • Non-CD
      • Immunology
      • Innate Immunity
      • Cytokines
      • Other
      • Signal Transduction
      • Growth Factors/Hormones
      • Insulin / Insulin-like
      • Neuroscience
      • Neurology process
      • Metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Recombinant human Visfatin protein (ab50134)
    • Recombinant Protein

      • Recombinant human Visfatin protein (ab198090)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab24149 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Pig
    Application Abreviews Notes
    WB
    1/500. Detects a band of approximately 52 kDa.
    Notes
    WB
    1/500. Detects a band of approximately 52 kDa.

    Target

    • Function

      Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
    • Tissue specificity

      Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
    • Pathway

      Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
    • Sequence similarities

      Belongs to the NAPRTase family.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P43490 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10135 Human
      • Omim: 608764 Human
      • SwissProt: P43490 Human
      • SwissProt: Q52I78 Pig
      • Unigene: 489615 Human
      • Alternative names

        • 1110035O14Rik antibody
        • AI314458 antibody
        • AI480535 antibody
        • DKFZP666B131 antibody
        • EC 2.4.2.12 antibody
        • MGC117256 antibody
        • NAmPRTase antibody
        • Nampt antibody
        • NAMPT_HUMAN antibody
        • Nicotinamide phosphoribosyltransferase antibody
        • PBEF 1 antibody
        • PBEF antibody
        • PBEF1 antibody
        • Pre B cell colony enhancing factor 1 antibody
        • Pre B cell colony enhancing factor antibody
        • Pre B cell enhancing factor antibody
        • Pre-B cell-enhancing factor antibody
        • Pre-B-cell colony-enhancing factor 1 antibody
        • VF antibody
        • Visfatin antibody
        see all

      Images

      • Western blot - Anti-Visfatin antibody (ab24149)
        Western blot - Anti-Visfatin antibody (ab24149)
        Anti-Visfatin antibody (ab24149) + Human adipose tissue lysate

        Observed band size: 52 kDa
        why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (4)

      Publishing research using ab24149? Please let us know so that we can cite the reference in this datasheet.

      ab24149 has been referenced in 4 publications.

      • Oppenheimer H  et al. Set7/9 impacts COL2A1 expression through binding and repression of SirT1 histone deacetylation. J Bone Miner Res 29:348-60 (2014). PubMed: 23873758
      • Naesens L  et al. Role of human hypoxanthine guanine phosphoribosyltransferase in activation of the antiviral agent T-705 (favipiravir). Mol Pharmacol 84:615-29 (2013). PubMed: 23907213
      • Mayi TH  et al. Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages. FEBS J 277:3308-20 (2010). WB ; Human . PubMed: 20608974
      • Tan BK  et al. In vivo and ex vivo regulation of visfatin production by leptin in human and murine adipose tissue: role of mitogen-activated protein kinase and phosphatidylinositol 3-kinase signaling pathways. Endocrinology 150:3530-9 (2009). WB ; Human, Mouse . PubMed: 19389835

      Customer reviews and Q&As

      Show All Reviews Q&A
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      ELISA abreview for Anti-Visfatin antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      ELISA
      Sample
      Mouse Recombinant protein (NA)
      Specification
      NA
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
      Type
      Direct
      Read More

      Dr. Mona Dvir-Ginzberg

      Verified customer

      Submitted Jan 14 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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