- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-Visfatin antibody
See all Visfatin primary antibodies -
DescriptionRabbit polyclonal to Visfatin
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Pig
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Immunogen
Synthetic peptide:
SFDEIRKNAQ LNIELEAAHH
, corresponding to C terminal amino acids 472-491 of Human PBEF. -
Positive control
- Purchase matching WB positive control:Recombinant Human Visfatin protein
- Human adipose tissue lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab24149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | 1/500. Detects a band of approximately 52 kDa. |
Target
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FunctionCatalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
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Tissue specificityExpressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
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PathwayCofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
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Sequence similaritiesBelongs to the NAPRTase family.
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Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 10135 Human
- Entrez Gene: 59027 Mouse
- Omim: 608764 Human
- SwissProt: P43490 Human
- SwissProt: Q99KQ4 Mouse
- SwissProt: Q52I78 Pig
- Unigene: 489615 Human
- Unigene: 202727 Mouse
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Alternative names
- 1110035O14Rik antibody
- AI314458 antibody
- AI480535 antibody
see all
Images
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Western blot - Anti-Visfatin antibody (ab24149)Anti-Visfatin antibody (ab24149) + Human adipose tissue lysate
Observed band size: 52 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody (ab24149)ICC/IF image of ab24149 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24149, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
This product has been referenced in:
- Naesens L et al. Role of human hypoxanthine guanine phosphoribosyltransferase in activation of the antiviral agent T-705 (favipiravir). Mol Pharmacol 84:615-29 (2013). Read more (PubMed: 23907213) »
- Mayi TH et al. Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages. FEBS J 277:3308-20 (2010). WB ; Human . Read more (PubMed: 20608974) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"