Anti-Visfatin antibody (ab24149)
Key features and details
- Rabbit polyclonal to Visfatin
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Visfatin antibody
See all Visfatin primary antibodies -
Description
Rabbit polyclonal to Visfatin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig
-
Immunogen
Synthetic peptide:
SFDEIRKNAQ LNIELEAAHH
, corresponding to C terminal amino acids 472-491 of Human PBEF. -
Positive control
- Purchase matching WB positive control:Recombinant Human Visfatin protein
- WB: U-87 MG, PC3, A431, HCT 116, Jurkat, Human adipose tissue ICC: U-87 MG
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
|
| WB |
1/500. Detects a band of approximately 52 kDa.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
WB
1/500. Detects a band of approximately 52 kDa. |
Target
-
Function
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. -
Tissue specificity
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. -
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1. -
Sequence similarities
Belongs to the NAPRTase family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 10135 Human
- Omim: 608764 Human
- SwissProt: P43490 Human
- SwissProt: Q52I78 Pig
- Unigene: 489615 Human
-
Alternative names
- 1110035O14Rik antibody
- AI314458 antibody
- AI480535 antibody
see all
Images
-
Western blot - Anti-Visfatin antibody (ab24149)All lanes : Anti-Visfatin antibody (ab24149) at 1/250 dilution
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with skimmed milk
Lane 2 : PC3 (Human prostate adenocarcinoma cell line) whole cell lysate with skimmed milk
Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate with skimmed milk
Lane 4 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate with skimmed milk
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with skimmed milk
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution
Additional bands at: ~55 kDa. We are unsure as to the identity of these extra bands.Detection: chemiluminescence
-
Western blot - Anti-Visfatin antibody (ab24149)Anti-Visfatin antibody (ab24149) + Human adipose tissue lysate
Observed band size: 52 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody (ab24149)Immunofluorescent analysis of 70% confluent log phase U87MG cells, 4% paraformaldehyde fixed and permeabilized with 0.1% Triton™ X-100, and blocked with 5% BSA for 1 hour at room temperature. ab24149 labeling Vistafin with 1 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Datasheets and documents
-
Datasheet download
References (4)
ab24149 has been referenced in 4 publications.
- Oppenheimer H et al. Set7/9 impacts COL2A1 expression through binding and repression of SirT1 histone deacetylation. J Bone Miner Res 29:348-60 (2014). PubMed: 23873758
- Naesens L et al. Role of human hypoxanthine guanine phosphoribosyltransferase in activation of the antiviral agent T-705 (favipiravir). Mol Pharmacol 84:615-29 (2013). PubMed: 23907213
- Mayi TH et al. Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages. FEBS J 277:3308-20 (2010). WB ; Human . PubMed: 20608974
- Tan BK et al. In vivo and ex vivo regulation of visfatin production by leptin in human and murine adipose tissue: role of mitogen-activated protein kinase and phosphatidylinositol 3-kinase signaling pathways. Endocrinology 150:3530-9 (2009). WB ; Human, Mouse . PubMed: 19389835
