Key features and details
- Rabbit polyclonal to Visfatin
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Visfatin antibody
See all Visfatin primary antibodies
DescriptionRabbit polyclonal to Visfatin
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig
Synthetic peptide aa 400-450. The exact sequence is proprietary.
Database link: P43490
- IP: HeLa whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Use at 2-5 µg/mg of lysate.
FunctionCatalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
Tissue specificityExpressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
PathwayCofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
Sequence similaritiesBelongs to the NAPRTase family.
- Information by UniProt
- 1110035O14Rik antibody
- AI314458 antibody
- AI480535 antibody
Visfatin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264145 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264145 at 1 µg/ml.
Lane 1: ab264145 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264145 has not yet been referenced specifically in any publications.