Recombinant Anti-Visfatin antibody [BLR058F] (ab252558)
![Western blot - Anti-Visfatin antibody [BLR058F] (ab252558)](https://www.abcam.com/ps/products/252/ab252558/Images/ab252558-343257-Rabbit-monoclonal-BLR058F-to-Visfatin-western-blot-RKO-MCF7-K562-GaMG-HEK293T-HeLa-U2OS-LNCaP-HepG2-Jurkat-NIH3T3-mouse-human.jpg "Western blot - Anti-Visfatin antibody [BLR058F] (ab252558)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR058F] to Visfatin
- Suitable for: IP, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Visfatin antibody [BLR058F]
See all Visfatin primary antibodies -
Description
Rabbit monoclonal [BLR058F] to Visfatin -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Visfatin aa 400-450. The exact sequence is proprietary. NP_005737.1
Database link: P43490 -
Positive control
- IHC-P: Human breast carcinoma and ovarian carcinoma tissue. ICC: NCI-H460. WB: RKO, MCF7, K562, GaMG, HEK-293T, HeLa, U-2 OS, LNCaP, HepG2, Jurkat and NIH/3T3 whole cell lysate. IP: Visfatin IP in HEK-293T whole cell lysate.
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Borate buffered saline -
Concentration information loading... -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR058F -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
5 µl/1 mg lysate |
|
| ICC/IF |
1/100 - 1/500.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. |
|
| WB |
1/1000. Predicted molecular weight: 55 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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IP
Use at an assay dependent concentration. 5 µl/1 mg lysate |
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ICC/IF
1/100 - 1/500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. |
|
WB
1/1000. Predicted molecular weight: 55 kDa. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. -
Tissue specificity
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. -
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1. -
Sequence similarities
Belongs to the NAPRTase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10135 Human
- Entrez Gene: 59027 Mouse
- Omim: 608764 Human
- SwissProt: P43490 Human
- SwissProt: Q99KQ4 Mouse
- Unigene: 489615 Human
- Unigene: 202727 Mouse
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Alternative names
- 1110035O14Rik antibody
- AI314458 antibody
- AI480535 antibody
see all
Images
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Western blot - Anti-Visfatin antibody [BLR058F] (ab252558)All lanes : Anti-Visfatin antibody [BLR058F] (ab252558) at 1/1000 dilution
Lane 1 : RKO (Human cell line) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : GaMG (Human cell line) whole cell lysate
Lane 5 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 7 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 8 : LNCaP (Human prostate cancer cell line) whole cell lysate
Lane 9 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 10 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 11 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 55 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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![Immunoprecipitation - Anti-Visfatin antibody [BLR058F] (ab252558)](https://www.abcam.com/ps/products/252/ab252558/Images/ab252558-343256-Rabbit-monoclonal-BLR058F-to-Visfatin-immunoprecipitation-HEK293T-human.jpg)
Immunoprecipitation - Anti-Visfatin antibody [BLR058F] (ab252558)Visfatin was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab252558 used for IP at 5 µl per reaction. For WB 1/1000 dilution.Lane 1: ab252558 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293Twhole cell lysate.Chemiluminescence detection: 75 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558)](https://www.abcam.com/ps/products/252/ab252558/Images/ab252558-343255-Rabbit-monoclonal-BLR058F-to-Visfatin-immunohistochemistry-ovarian-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for Visfatin with ab252558 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558)](https://www.abcam.com/ps/products/252/ab252558/Images/ab252558-343254-Rabbit-monoclonal-BLR058F-to-Visfatin-immunohistochemistry-breast-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Visfatin with ab252558 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
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![Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [BLR058F] (ab252558)](https://www.abcam.com/ps/products/252/ab252558/Images/ab252558-343253-Rabbit-monoclonal-BLR058F-to-Visfatin-immunocytochemistry-NCIH460-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [BLR058F] (ab252558)FFPE section of NCI-H460 (Human lung cancer cell line) cells labeling Visfatin using ab252558 at a 1/250 dilution in ICC.
Secondary: HRP-conjugated goat anti-rabbit IgG.
Substrate: DAB.
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![Anti-Visfatin antibody [BLR058F] (ab252558)](https://www.abcam.com/ps/products/252/ab252558/Images/ab252558-6-benefits-of-recombinant-antibodies.png)
Anti-Visfatin antibody [BLR058F] (ab252558)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab252558 has not yet been referenced specifically in any publications.