Recombinant
RabMAb

Recombinant Anti-Visfatin antibody [EPR21980] (ab236874)

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Overview

  • Product name

    Anti-Visfatin antibody [EPR21980]
    See all Visfatin primary antibodies

  • Description

    Rabbit monoclonal [EPR21980] to Visfatin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Visfatin aa 300-400. The exact sequence is proprietary.
    Database link: P43490

  • Positive control

    • WB: K562, Raji, HeLa, HT-1080, PC-3, MCF7, HCT 116, C6, PC-12 and Raw 264.7 whole cell lysate; Human fetal kidney lysate. IHC-P: Human bladder cancer and breast cancer tissue; Mouse kidney tissue; Rat liver tissue. ICC/IF: HCT 116 and K562 cells. Flow: K562 and HCT 116 cells. IP: K562 whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 55 kDa.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
ICC/IF 1/100.
Flow Cyt 1/500.
IP 1/30.

Target

  • Function

    Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.

  • Tissue specificity

    Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.

  • Pathway

    Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.

  • Sequence similarities

    Belongs to the NAPRTase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 1110035O14Rik antibody
    • AI314458 antibody
    • AI480535 antibody
    • DKFZP666B131 antibody
    • EC 2.4.2.12 antibody
    • MGC117256 antibody
    • NAmPRTase antibody
    • Nampt antibody
    • NAMPT_HUMAN antibody
    • Nicotinamide phosphoribosyltransferase antibody
    • PBEF 1 antibody
    • PBEF antibody
    • PBEF1 antibody
    • Pre B cell colony enhancing factor 1 antibody
    • Pre B cell colony enhancing factor antibody
    • Pre B cell enhancing factor antibody
    • Pre-B cell-enhancing factor antibody
    • Pre-B-cell colony-enhancing factor 1 antibody
    • VF antibody
    • Visfatin antibody
    see all

Images

  • Western blot - Anti-Visfatin antibody [EPR21980] (ab236874)
    Western blot - Anti-Visfatin antibody [EPR21980] (ab236874)
    All lanes : Anti-Visfatin antibody [EPR21980] (ab236874) at 1/1000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
    Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 4 : HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate at 20 µg
    Lane 5 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 6 : Human fetal kidney lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 55 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST 

    Exposure times:
    Lanes 1-2: 26 seconds
    Lanes 3-5: 92 seconds
    Lane 6: 3 minutes

    Lane 6 was developed using a higher sensitivity ECL substrate.

    The molecular weight observed is consistent with what has been described in the literature (PMID:25368373, PMID:12782293).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21980] (ab236874)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21980] (ab236874)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Visfatin using ab236874 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining in human bladder cancer (PMID:26396920) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [EPR21980] (ab236874)
    Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [EPR21980] (ab236874)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labeling Visfatin (Green) with ab236874 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in HCT 116 cell line is observed. The nuclear counterstain is DAPI (Blue). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as the counterstain (Red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Alexa-Fluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

  • Immunoprecipitation - Anti-Visfatin antibody [EPR21980] (ab236874)
    Immunoprecipitation - Anti-Visfatin antibody [EPR21980] (ab236874)

    Visfatin was immunoprecipitated from 0.35 mg of K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab236874 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236874 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: K562 whole cell lysate 10 μg (input).
    Lane 2: ab236874 IP in K562 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236874 in K562 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Flow Cytometry - Anti-Visfatin antibody [EPR21980] (ab236874)
    Flow Cytometry - Anti-Visfatin antibody [EPR21980] (ab236874)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cell line labeling Visfatin with ab236874 at 1/500 dilution (Red) compared with the Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody, Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-Visfatin antibody [EPR21980] (ab236874)
    Flow Cytometry - Anti-Visfatin antibody [EPR21980] (ab236874)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized K562 (human chronic myelogenous leukemia lymphoblast) cell line labeling Visfatin with ab236874 at 1/500 dilution (Red) compared with the Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody, Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21980] (ab236874)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21980] (ab236874)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Visfatin using ab236874 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining in rat liver (PMID: 29104634; PMID:24603648) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21980] (ab236874)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21980] (ab236874)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Visfatin using ab236874 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining in mouse kidney (PMID: 29104634; PMID:24287278) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21980] (ab236874)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21980] (ab236874)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Visfatin using ab236874 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining in human breast cancer (PMID:21784959) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Visfatin antibody [EPR21980] (ab236874)
    Western blot - Anti-Visfatin antibody [EPR21980] (ab236874)
    All lanes : Anti-Visfatin antibody [EPR21980] (ab236874) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 2 : HCT 116 (human colorectal carcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 3 : HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate at 20 µg
    Lane 4 : C6 (rat glial tumor glial cell), whole cell lysate at 20 µg
    Lane 5 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg
    Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 55 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time:
    Lanes 1-3: 40 seconds
    Lanes 4-5: 3 minutes
    Lane 6: 15 seconds

    The molecular weight observed is consistent with what has been described in the literature (PMID:25368373, PMID:12782293).

  • Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [EPR21980] (ab236874)
    Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [EPR21980] (ab236874)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (human chronic myelogenous leukemia lymphoblast) cells labeling Visfatin (Green) with ab236874 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in K562 cell line is observed. The nuclear counterstain is DAPI (Blue). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as the counterstain (Red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Alexa-Fluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

References

ab236874 has not yet been referenced specifically in any publications.

Publishing research using ab236874? Please let us know so that we can cite the reference in this datasheet.

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