Recombinant Anti-Visfatin antibody [EPR21984] (ab236873)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21984] to Visfatin
- Suitable for: WB, IHC-P, IP, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Visfatin antibody [EPR21984]
See all Visfatin primary antibodies -
Description
Rabbit monoclonal [EPR21984] to Visfatin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IP, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, HT-1080, PC-3, NCI-H460, K562, MCF7, HCT 116, PC-12 and C2C12 whole cell lysates; Human breast cancer, fetal brain, heart and fetal kidney lysates. IHC-P: Human bladder cancer and breast cancer tissues; Mouse kidney tissue; Rat liver tissue. Flow Cyt (intra): K562 and HC T116 cells. IP: HCT 116 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21984 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 55 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/30.
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Flow Cyt (Intra) |
1/500.
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Notes |
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WB
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 55 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/30. |
Flow Cyt (Intra)
1/500. |
Target
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Function
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. -
Tissue specificity
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. -
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1. -
Sequence similarities
Belongs to the NAPRTase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10135 Human
- Entrez Gene: 59027 Mouse
- Entrez Gene: 297508 Rat
- Omim: 608764 Human
- SwissProt: P43490 Human
- SwissProt: Q99KQ4 Mouse
- SwissProt: Q80Z29 Rat
- Unigene: 489615 Human
see all -
Alternative names
- 1110035O14Rik antibody
- AI314458 antibody
- AI480535 antibody
see all
Images
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All lanes : Anti-Visfatin antibody [EPR21984] (ab236873) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 20 µg
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
Lane 4 : C2C12 (mouse myoblast cell line) whole cell lysate at 20 µg
Lane 5 : Human fetal brain lysate at 10 µg
Lane 6 : Human heart lysate at 10 µg
Lane 7 : Human fetal kidney lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 55 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:25368373, PMID:12782293).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in human bladder cancer (PMID:26396920) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling Visfatin with ab236873 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody
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Visfatin was immunoprecipitated from 0.35 mg HCT 116 (human colorectal carcinoma cell line) whole cell lysate with ab236873 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236873 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: HTC 116 whole cell lysate 10 µg (Input).
Lane 2: ab236873 IP in HCT 116 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236873 in HCT 116 whole cell lysate (-).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second. -
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HCT 116 (human colorectal carcinoma cell line) cell line labeling Visfatin with ab236873 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in rat liver (PMID: 29104634; PMID:24603648) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in mouse kidney (PMID: 29104634; PMID:24287278) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in human breast cancer(PMID:21784959) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Visfatin antibody [EPR21984] (ab236873) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 2 : HT1080 (human fibrosarcoma cell line) whole cell lysate at 20 µg
Lane 3 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 4 : NCI-H460 (human lung cancer cell line) whole cell lysate at 20 µg
Lane 5 : Human breast cancer lysate at 20 µg
Lane 6 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 55 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1-3: 37 seconds; Lanes 4-5: 8 seconds; Lane 6: 26 seconds.
Blocking and diluting buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:25368373, PMID:12782293).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab236873 has not yet been referenced specifically in any publications.