Recombinant
RabMAb

Recombinant Anti-Visfatin antibody [EPR21984] (ab236873)

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Overview

  • Product name

    Anti-Visfatin antibody [EPR21984]
    See all Visfatin primary antibodies

  • Description

    Rabbit monoclonal [EPR21984] to Visfatin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein within Human Visfatin aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P43490

  • Positive control

    • WB: Jurkat, HT-1080, PC-3, NCI-H460, K562, MCF7, HCT 116, PC-12 and C2C12 whole cell lysates; Human breast cancer, fetal brain, heart and fetal kidney lysates. IHC-P: Human bladder cancer and breast cancer tissues; Mouse kidney tissue; Rat liver tissue. Flow Cyt: K562 and HC T116 cells. IP: HCT 116 cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/500.
IP 1/30.

Target

  • Function

    Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.

  • Tissue specificity

    Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.

  • Pathway

    Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.

  • Sequence similarities

    Belongs to the NAPRTase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 1110035O14Rik antibody
    • AI314458 antibody
    • AI480535 antibody
    • DKFZP666B131 antibody
    • EC 2.4.2.12 antibody
    • MGC117256 antibody
    • NAmPRTase antibody
    • Nampt antibody
    • NAMPT_HUMAN antibody
    • Nicotinamide phosphoribosyltransferase antibody
    • PBEF 1 antibody
    • PBEF antibody
    • PBEF1 antibody
    • Pre B cell colony enhancing factor 1 antibody
    • Pre B cell colony enhancing factor antibody
    • Pre B cell enhancing factor antibody
    • Pre-B cell-enhancing factor antibody
    • Pre-B-cell colony-enhancing factor 1 antibody
    • VF antibody
    • Visfatin antibody
    see all

Images

  • Western blot - Anti-Visfatin antibody [EPR21984] (ab236873)
    Western blot - Anti-Visfatin antibody [EPR21984] (ab236873)
    All lanes : Anti-Visfatin antibody [EPR21984] (ab236873) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 20 µg
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
    Lane 4 : C2C12 (mouse myoblast cell line) whole cell lysate at 20 µg
    Lane 5 : Human fetal brain lysate at 10 µg
    Lane 6 : Human heart lysate at 10 µg
    Lane 7 : Human fetal kidney lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 55 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:25368373, PMID:12782293).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in human bladder cancer (PMID:26396920) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Flow Cytometry - Anti-Visfatin antibody [EPR21984] (ab236873)
    Flow Cytometry - Anti-Visfatin antibody [EPR21984] (ab236873)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling Visfatin with ab236873 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody

  • Immunoprecipitation - Anti-Visfatin antibody [EPR21984] (ab236873)
    Immunoprecipitation - Anti-Visfatin antibody [EPR21984] (ab236873)

    Visfatin was immunoprecipitated from 0.35 mg HCT 116 (human colorectal carcinoma cell line) whole cell lysate with ab236873 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236873 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: HTC 116 whole cell lysate 10 µg (Input).
    Lane 2: ab236873 IP in HCT 116 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236873 in HCT 116 whole cell lysate (-).


    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

  • Flow Cytometry - Anti-Visfatin antibody [EPR21984] (ab236873)
    Flow Cytometry - Anti-Visfatin antibody [EPR21984] (ab236873)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HCT 116 (human colorectal carcinoma cell line) cell line labeling Visfatin with ab236873 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in rat liver (PMID: 29104634; PMID:24603648) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in mouse kidney (PMID: 29104634; PMID:24287278) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21984] (ab236873)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Visfatin with ab236873 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in human breast cancer(PMID:21784959) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Western blot - Anti-Visfatin antibody [EPR21984] (ab236873)
    Western blot - Anti-Visfatin antibody [EPR21984] (ab236873)
    All lanes : Anti-Visfatin antibody [EPR21984] (ab236873) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
    Lane 2 : HT1080 (human fibrosarcoma cell line) whole cell lysate at 20 µg
    Lane 3 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 4 : NCI-H460 (human lung cancer cell line) whole cell lysate at 20 µg
    Lane 5 : Human breast cancer lysate at 20 µg
    Lane 6 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 55 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?



    Exposure time : Lanes 1-3: 37 seconds; Lanes 4-5: 8 seconds; Lane 6: 26 seconds.

    Blocking and diluting buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:25368373, PMID:12782293).

References

ab236873 has not yet been referenced specifically in any publications.

Publishing research using ab236873? Please let us know so that we can cite the reference in this datasheet.

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