Recombinant
RabMAb

Recombinant Anti-VISTA antibody [EPR21050] (ab230950)

Overview

  • Product name

    Anti-VISTA antibody [EPR21050]
    See all VISTA primary antibodies
  • Description

    Rabbit monoclonal [EPR21050] to VISTA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human VISTA aa 1-350. The exact sequence is proprietary.
    Database link: Q9H7M9

  • Positive control

    • WB: Human thymus, fetal spleen and fetal lung lysate. DU154 whole cell lysate. IHC-P: Human lung cancer and spleen tissue. IP: DU154 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21050
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 34 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.

Target

  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B7 H5 antibody
    • B7H5 antibody
    • C10orf54 antibody
    • chromosome 10 open reading frame 54 antibody
    • DD1alpha antibody
    • GI24 antibody
    • GI24_HUMAN antibody
    • PD-1H antibody
    • PDCD1 homolog antibody
    • Platelet receptor Gi24 antibody
    • PP2135 antibody
    • sisp 1 antibody
    • SISP1 antibody
    • stress induced secreted protein 1 antibody
    • UNQ730/PRO1412 antibody
    • V domain Ig suppressor of T cell activation antibody
    • V set domain containing immunoregulatory receptor antibody
    • V set immunoregulatory receptor antibody
    • V type immunoglobulin domain containing suppressor of T cell activation antibody
    • VISTA antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling VISTA with ab230950 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Membranous and cytoplasmic staining in tumor-infiltrating leukocytes of human lung cancer (PMID: 28236118, PMID: 28507801) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • All lanes : Anti-VISTA antibody [EPR21050] (ab230950) at 1/1000 dilution

    Lane 1 : Human fetal spleen lysate
    Lane 2 : Human thymus lysate
    Lane 3 : DU145 (Human prostate carcinoma epithelial cell) whole cell lysate
    Lane 4 : Human fetal lung lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 32 seconds; Lanes 3-4: 6 seconds.

    The 50-60 kda band observed is the glycosylated form of VISTA, consistent with what has been described in the literature (PMID: 29216931).

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling VISTA with ab230950 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Membranous and cytoplasmic staining in leukocytes of human spleen (PMID: 21383057, PMID: 24691993) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • VISTA was immunoprecipitated from 0.35 mg DU145 (Human prostate carcinoma epithelial cell) whole cell lysate with ab230950 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230950 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
    Lane 1: DU154 whole cell lysate 10 µg (Input).
    Lane 2: ab230950 IP in DU154 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230950 in DU154 whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

References

ab230950 has not yet been referenced specifically in any publications.

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