Recombinant
RabMAb

Recombinant Anti-VISTA antibody [EPR21050] - BSA and Azide free (ab238886)

Overview

  • Product name

    Anti-VISTA antibody [EPR21050] - BSA and Azide free
    See all VISTA primary antibodies
  • Description

    Rabbit monoclonal [EPR21050] to VISTA - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human VISTA aa 1-350. The exact sequence is proprietary.
    Database link: Q9H7M9

  • Positive control

    • IHC-P: Human spleen tissue.
  • General notes

    Ab238886 is the carrier-free version of ab230950. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238886 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21050
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab238886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Target

  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B7 H5 antibody
    • B7H5 antibody
    • C10orf54 antibody
    • chromosome 10 open reading frame 54 antibody
    • DD1alpha antibody
    • GI24 antibody
    • GI24_HUMAN antibody
    • PD-1H antibody
    • PDCD1 homolog antibody
    • Platelet receptor Gi24 antibody
    • PP2135 antibody
    • sisp 1 antibody
    • SISP1 antibody
    • stress induced secreted protein 1 antibody
    • UNQ730/PRO1412 antibody
    • V domain Ig suppressor of T cell activation antibody
    • V set domain containing immunoregulatory receptor antibody
    • V set immunoregulatory receptor antibody
    • V type immunoglobulin domain containing suppressor of T cell activation antibody
    • VISTA antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling VISTA with ab230950 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Membranous and cytoplasmic staining in tumor-in?ltrating leukocytes of human lung cancer (PMID: 28236118, PMID: 28507801) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230950).

  • VISTA was immunoprecipitated from 0.35 mg DU145 (Human prostate carcinoma epithelial cell) whole cell lysate with ab230950 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230950 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
    Lane 1: DU154 whole cell lysate 10 µg (Input).
    Lane 2: ab230950 IP in DU154 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230950 in DU154 whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230950).

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling VISTA with ab230950 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Membranous and cytoplasmic staining in leukocytes of human spleen (PMID: 21383057, PMID: 24691993) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab230950).

References

ab238886 has not yet been referenced specifically in any publications.

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