Recombinant Anti-Vitamin B2 antibody [EPR20853-141] (ab218125)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20853-141] to Vitamin B2
- Suitable for: Dot blot
Related conjugates and formulations
Overview
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Product name
Anti-Vitamin B2 antibody [EPR20853-141]
See all Vitamin B2 primary antibodies -
Description
Rabbit monoclonal [EPR20853-141] to Vitamin B2 -
Host species
Rabbit -
Tested applications
Suitable for: Dot blotmore details -
Immunogen
Chemical/ Small Molecule corresponding to Vitamin B2 conjugated to bovine serum albumin.
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Positive control
- Dot blot: Ovalbumin (OVA)-Riboflavin.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20853-141 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
1/1000.
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Notes |
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Dot blot
1/1000. |
Target
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Relevance
Riboflavin (vitamin B2) is manufactured in the body by the intestinal flora and is easily absorbed, although very small quantities are stored, so there is a constant need for this vitamin. It is required by the body to use oxygen and the metabolism of amino acids, fatty acids, and carbohydrates. Riboflavin is further needed to activate vitamin B6 (pyridoxine), helps to create niacin and assists the adrenal gland. It may be used for red blood cell formation, antibody production, cell respiration, and growth. It eases watery eye fatigue and may be helpful in the prevention and treatment of cataracts. Vitamin B2 is required for the health of the mucus membranes in the digestive tract and helps with the absorption of iron and vitamin B6. -
Cellular localization
Secreted -
Alternative names
- Flavin Mononucleotide antibody
- FMN antibody
- Riboflavin antibody
Images
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Dot blot analysis of Vitamin B12 using ab218125 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.
Lane 1: Ovalbumin (OVA)-Riboflavin
Lane 2: OVA-Folic acid (carboxyl conjugation)
Lane 3: OVA-Folic acid (amino conjugation)
Lane 4: OVA-Histamine
Exposure time: 3 minutes.
Blocking/Diluting buffer: 5% NFDM/TBST
Folic acid and Histamine are the analogue of Riboflavin.
Ovalbumin (OVA)-conjugated small molecules are kindly provided by Dr Rao Yi.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab218125 has not yet been referenced specifically in any publications.