Product nameAnti-Vitamin B2 antibody [EPR20853-141]
See all Vitamin B2 primary antibodies
DescriptionRabbit monoclonal [EPR20853-141] to Vitamin B2
Tested applicationsSuitable for: Dot blotmore details
Chemical/ Small Molecule corresponding to Vitamin B2 conjugated to bovine serum albumin.
- Dot blot: Ovalbumin (OVA)-Riboflavin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab218125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceRiboflavin (vitamin B2) is manufactured in the body by the intestinal flora and is easily absorbed, although very small quantities are stored, so there is a constant need for this vitamin. It is required by the body to use oxygen and the metabolism of amino acids, fatty acids, and carbohydrates. Riboflavin is further needed to activate vitamin B6 (pyridoxine), helps to create niacin and assists the adrenal gland. It may be used for red blood cell formation, antibody production, cell respiration, and growth. It eases watery eye fatigue and may be helpful in the prevention and treatment of cataracts. Vitamin B2 is required for the health of the mucus membranes in the digestive tract and helps with the absorption of iron and vitamin B6.
- Flavin Mononucleotide antibody
- FMN antibody
- Riboflavin antibody
Dot blot analysis of Vitamin B12 using ab218125 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.
Lane 1: Ovalbumin (OVA)-Riboflavin
Lane 2: OVA-Folic acid (carboxyl conjugation)
Lane 3: OVA-Folic acid (amino conjugation)
Lane 4: OVA-Histamine
Exposure time: 3 minutes.
Blocking/Diluting buffer: 5% NFDM/TBST
Folic acid and Histamine are the analogue of Riboflavin.
Ovalbumin (OVA)-conjugated small molecules are kindly provided by Dr Rao Yi.
ab218125 has not yet been referenced specifically in any publications.