Overview

  • Product name
    Anti-Vitamin C antibody
  • Description
    Rat polyclonal to Vitamin C
  • Host species
    Rat
  • Specificity
    This antibody is specific for conjugated Vitamin C, it does not recognize free Vitamin C.
  • Tested applications
    Suitable for: Electron Microscopymore details
  • Immunogen

    Chemical/ Small Molecule corresponding to Vitamin C conjugated to Bovine Serum Albumin (BSA).

Properties

Applications

Our Abpromise guarantee covers the use of ab37020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy Use at an assay dependent concentration. PubMed: 20872269

Target

  • Relevance
    Vitamin C (L ascorbic acid and its reduced form, dehydroascorbic acid) is a water-soluble nutrient and vitamin essential for life and for maintaining optimal health. It's best-defined function is as a cofactor for the enzyme required in the hydroxylation of proline and lysine in collagen formation. Vitamin C is an antioxidant and is used by the body for many purposes. Almost all animals and plants synthesize their own vitamin C, with a few exceptions, such as humans and a small number of other animals, including, apes, guinea pigs, the red-vented bulbul, a fruit-eating bat and a species of trout. No bodily organ stores ascorbate as a primary function, and so the body soon depletes itself of ascorbate if fresh supplies are not consumed through the digestive system, eventually leading to the deficiency disease known as scurvy. There is continuing debate within the scientific community over the amount and frequency of intake of Vitamin C for maintaining optimal health in humans.
  • Alternative names
    • 3 oxo L gulofuranolactone antibody
    • Ascorbic acid antibody
    • Dehydroascorbic acid antibody
    • Salt ascorbate antibody
    • Vitamin C antibody
    see all

References

This product has been referenced in:
  • Zechmann B  et al. Immunocytochemical determination of the subcellular distribution of ascorbate in plants. Planta 233:1-12 (2011). Electron Microscopy . Read more (PubMed: 20872269) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply.

ab75764 is the molecule Vitamin C (http://en.wikipedia.org/wiki/Vitamin_C)itself and could be used a positive control for your customers experiments or as standardwhen doing an ELISA.

We have ab37020 (anti Vitamin C (conjugated) antibody) in our catalog. This would be suitable to measure/detect Vitamin C in an ELISA.

Please let your customer review the datasheet. It also has a link to the recommended ELISA protocol for ab37020.

https://www.abcam.com/index.html?datasheet=37020 (or use the following: https://www.abcam.com/index.html?datasheet=37020).

How many tests can be done is dependent on the dilution that is used and also the volume per test.

Please also let your customer know about our protocols page, where also protocols for ELISAs can be found:

https://www.abcam.com/index.html?pageconfig=popular_protocols

I hope this information is helpful for your customer.

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Answer

Thank you for passing on this enquiry from the customer. This antibody (ab37020) will not recognize unconjugated ascorbic acid as the ascorbic acid molecule is too small. I can suggest trying the following ELISA protocol which has been forwarded to me by the laboratory: 1. Coat ELISA plates with conjugated ascorbic acid (15µg/ml) with a solution of sodium carbonate buffer 0.05M (pH 9.6). Incubate 16 hours at 4°C. 2. Incubate the plates with PBS (pH 7.3) containing 1% BSA and 0.05%Tween 20 for one hour at 37°C. 3. Wash with PBS Tween (three times). 4. Dilute preabsorbed ascorbic acid antiserum (1/2,000-1/5,000) in PBS tween containing 1% BSA and 5% glycerol. Add 200µl per well and incubate 2 hours at 37°C. 5. Wash with PBS Tween (three times). 6. Add 200µl per well of peroxidase-labeled goat anti-rat antibody diluted PBS containing 1% BSA and 0.5% Tween. Incubate one hour at 37°C. 7. Wash wells with PBS Tween. 8. Develop the peroxidase by incubating 200µl per well with citrate 0.1M/phosphate 0.2M (pH 5) solution containing 0.4% of OPD and 0.03% of hydrogen peroxide for ten minutes in the dark. Stop the reaction by the addition of 50µl of 2M HCl. 9. Measure the optical density at 492nm, to obtain the different values (IC 50). I hope this information is helpful. Should the customer have any further questions, please do not hesitate to contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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