Question (60130) | Anti-Vitamin D Binding protein antibody [2G2] (ab23480)

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Question

Dear technical team,

I purchased ab23480, ab121060, ab90920 and would like to do sandwich ELISA.
I would like to know epitope sequences of ab23480 and ab121060. In addition, May I ask if ab23480 can be used as capture antibody and ab121063 (instead of ab121060) as detection antibody?

Answer

Thank you for contacting us.

Full length vitamin D binding protein is used as immunogen for ab121060, ab121063 and ab23480 but we are sorry to tell that we do not have a specific epitope mapping, down to the amino acid sequence, available. These antibodies have been tested in inhibition ELISA experiment, none of the antibodies inhibit each other. Hence, we believe that they do all react with different epitopes. However, as we have not tested ab23480 in sandwich ELISA with ab121063, we cannot tell whether ab23480 can be a suitable sandwich ELISA pair with ab121063, of which you have to test in your own experiment.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any further advice or information

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