Anti-Vitamin D Binding protein antibody (ab153922)
Key features and details
- Rabbit polyclonal to Vitamin D Binding protein
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Vitamin D Binding protein antibody
See all Vitamin D Binding protein primary antibodies -
Description
Rabbit polyclonal to Vitamin D Binding protein -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 211-448 of Human Vitamin D Binding protein.
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Positive control
- Jurkat, K562 and BCL-1 whole cell lysates; Human Cal27 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2638 Human
- Entrez Gene: 14473 Mouse
- Omim: 139200 Human
- SwissProt: P02774 Human
- SwissProt: P21614 Mouse
- Unigene: 418497 Human
- Unigene: 196595 Mouse
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Alternative names
- DBP antibody
- DBP/GC antibody
- GC antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Cal27 (human) xenograft labeling Vitamin D Binding protein with ab153922 at 1/500 dilution.
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Anti-Vitamin D Binding protein antibody (ab153922) at 1/1000 dilution + Mouse BCL-1 whole cell lysate at 30 µg
Predicted band size: 53 kDa
10% SDS PAGE -
All lanes : Anti-Vitamin D Binding protein antibody (ab153922) at 1/1000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : K562 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
10% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab153922 has been referenced in 1 publication.
- Nabeshima Y et al. Simple method for large-scale production of macrophage activating factor GcMAF. Sci Rep 10:19122 (2020). PubMed: 33154460