Overview

  • Product name

    Anti-Vitamin D Binding protein antibody
    See all Vitamin D Binding protein primary antibodies
  • Description

    Rabbit polyclonal to Vitamin D Binding protein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Vitamin D Binding Protein.

    Read Abcam's proprietary immunogen policy (Peptide available as ab71146.)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: human liver and human ovary IHC-P: Human kidney FFPE tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab65636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Target

  • Function

    Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • DBP antibody
    • DBP/GC antibody
    • GC antibody
    • Gc globulin antibody
    • Gc-globulin antibody
    • GRD3 antibody
    • Group specific component antibody
    • Group specific component vitamin D binding protein antibody
    • Group-specific component antibody
    • hDBP antibody
    • VDB antibody
    • VDBG antibody
    • VDBP antibody
    • Vitamin D binding alpha globulin antibody
    • Vitamin D-binding protein antibody
    • VTDB_HUMAN antibody
    see all

Images

  • All lanes : Anti-Vitamin D Binding protein antibody (ab65636) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human ovary tissue lysate - total protein (ab30222)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 1 minute
  • IHC image of Vitamin D Binding Protein staining in human kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65636, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

  • ICC/IF image of ab65636 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65636, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Ackermann AM  et al. Integration of ATAC-seq and RNA-seq identifies human alpha cell and beta cell signature genes. Mol Metab 5:233-44 (2016). Human . Read more (PubMed: 26977395) »
  • Taniuchi K  et al. RUVBL1 directly binds actin filaments and induces formation of cell protrusions to promote pancreatic cancer cell invasion. Int J Oncol 44:1945-54 (2014). Read more (PubMed: 24728183) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
None
Permeabilization
Yes - 0,5% Triton X-100
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Eileen Dahlke

Verified customer

Submitted Aug 13 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate buffer, pH 6
Permeabilization
No
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Eileen Dahlke

Verified customer

Submitted Aug 13 2018

Application
Western blot
Sample
Pig Tissue lysate - whole (endometrial tissue lysate)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
25 µg
Specification
endometrial tissue lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jul 28 2015

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