Anti-Vitamin D Binding protein antibody (ab95469)
- Datasheet
- Specific References (1)
- Protocols
Overview
-
Product nameAnti-Vitamin D Binding protein antibody
See all Vitamin D Binding protein primary antibodies -
DescriptionGoat polyclonal to Vitamin D Binding protein
-
Host speciesGoat
-
Tested applicationsSuitable for: WBmore details
-
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog
-
Immunogen
Synthetic peptide:
ERGRDYEKNKVCK
, corresponding to internal sequence (near the N terminal) amino acids 18-30 of Human Vitamin D Binding protein (NP_000574.2) -
Positive control
- Human lung and ovary lysates
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesab95469 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab95469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa). |
Target
-
FunctionMultifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
-
Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Cellular localizationSecreted.
- Information by UniProt
-
Database links
- Entrez Gene: 2638 Human
- Entrez Gene: 14473 Mouse
- Entrez Gene: 24384 Rat
- Omim: 139200 Human
- SwissProt: P02774 Human
- SwissProt: P21614 Mouse
- SwissProt: P04276 Rat
- Unigene: 418497 Human
see all -
Alternative names
- DBP antibody
- DBP/GC antibody
- GC antibody
see all
Images
-
Western blot - Anti-Vitamin D Binding protein antibody (ab95469)Anti-Vitamin D Binding protein antibody (ab95469) at 1 µg/ml + Human lung lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Primary incubation was 1 hour.
Protocols
References
This product has been referenced in:
- Chacar F et al. Urinary Tamm-Horsfall protein, albumin, vitamin D-binding protein, and retinol-binding protein as early biomarkers of chronic kidney disease in dogs. Physiol Rep 5:N/A (2017). WB ; Dog . Read more (PubMed: 28576851) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"