Anti-Vitamin D Binding protein antibody (ab95469)

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Western blot - Anti-Vitamin D Binding protein antibody (ab95469)

    Key features and details

    • Goat polyclonal to Vitamin D Binding protein
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-Vitamin D Binding protein antibody
      See all Vitamin D Binding protein primary antibodies

    • Description

      Goat polyclonal to Vitamin D Binding protein

    • Host species

      Goat

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog

    • Immunogen

      Synthetic peptide:

      ERGRDYEKNKVCK

      , corresponding to internal sequence (near the N terminal) amino acids 18-30 of Human Vitamin D Binding protein (NP_000574.2)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Human lung and ovary lysates

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab95469 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 0.5 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).

    A 1 hour primary incubation is recommended for this product.

    Target

    • Function

      Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.

    • Sequence similarities

      Belongs to the ALB/AFP/VDB family.
      Contains 3 albumin domains.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • DBP antibody
      • DBP/GC antibody
      • GC antibody
      • Gc globulin antibody
      • Gc-globulin antibody
      • GRD3 antibody
      • Group specific component antibody
      • Group specific component vitamin D binding protein antibody
      • Group-specific component antibody
      • hDBP antibody
      • VDB antibody
      • VDBG antibody
      • VDBP antibody
      • Vitamin D binding alpha globulin antibody
      • Vitamin D-binding protein antibody
      • VTDB_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Vitamin D Binding protein antibody (ab95469)
      Western blot - Anti-Vitamin D Binding protein antibody (ab95469)
      Anti-Vitamin D Binding protein antibody (ab95469) at 1 µg/ml + Human lung lysate (in RIPA buffer) at 35 µg

      Developed using the ECL technique.

      Predicted band size: 53 kDa



      Primary incubation was 1 hour.

    References (1)

    Publishing research using ab95469? Please let us know so that we can cite the reference in this datasheet.

    ab95469 has been referenced in 1 publication.

    • Chacar F  et al. Urinary Tamm-Horsfall protein, albumin, vitamin D-binding protein, and retinol-binding protein as early biomarkers of chronic kidney disease in dogs. Physiol Rep 5:N/A (2017). WB ; Dog . PubMed: 28576851

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