Anti-Vitamin D Binding protein antibody [C1] (ab239733)
Key features and details
- Mouse monoclonal [C1] to Vitamin D Binding protein
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG2b
Overview
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Product name
Anti-Vitamin D Binding protein antibody [C1]
See all Vitamin D Binding protein primary antibodies -
Description
Mouse monoclonal [C1] to Vitamin D Binding protein -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse -
Immunogen
Recombinant fragment (His-tag) corresponding to Human Vitamin D Binding protein aa 200-400. N-terminal tag (Expressed in E.coli).
Database link: P04276 -
Positive control
- WB: Recombinant rat Vitamin D Binding protein; Rat serum. IHC-P: Rat pancreas tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.5 - 5 µg/ml.
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IHC-P |
Use a concentration of 5 - 30 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 5 µg/ml. |
IHC-P
Use a concentration of 5 - 30 µg/ml. |
Target
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Function
Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 14473 Mouse
- Entrez Gene: 24384 Rat
- SwissProt: P21614 Mouse
- SwissProt: P04276 Rat
- Unigene: 196595 Mouse
- Unigene: 1437 Rat
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Alternative names
- DBP antibody
- DBP/GC antibody
- GC antibody
see all
Images
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Paraffin-embedded rat pancreas tissue stained for Vitamin D binding protein using ab239733 at 40 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-Vitamin D Binding protein antibody [C1] (ab239733) at 3 µg/ml + Rat serum
Secondary
HRP-Linked Rabbit anti-Mouse IgG at 1/5000 dilution -
Anti-Vitamin D Binding protein antibody [C1] (ab239733) at 5 µg/ml + Recombinant rat Vitamin D Binding protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab239733 has not yet been referenced specifically in any publications.