Recombinant Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)


  • Product name

    Anti-Vitamin D Binding protein antibody [EP2923Y]
    See all Vitamin D Binding protein primary antibodies
  • Description

    Rabbit monoclonal [EP2923Y] to Vitamin D Binding protein
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Vitamin D Binding protein aa 250-350. The exact sequence is proprietary.

  • Positive control

    • Human liver tissue. Human plasma.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab81307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
    • Sequence similarities

      Belongs to the ALB/AFP/VDB family.
      Contains 3 albumin domains.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • DBP antibody
      • DBP/GC antibody
      • GC antibody
      • Gc globulin antibody
      • Gc-globulin antibody
      • GRD3 antibody
      • Group specific component antibody
      • Group specific component vitamin D binding protein antibody
      • Group-specific component antibody
      • hDBP antibody
      • VDB antibody
      • VDBG antibody
      • VDBP antibody
      • Vitamin D binding alpha globulin antibody
      • Vitamin D-binding protein antibody
      • VTDB_HUMAN antibody
      see all


    • Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307) at 1/20000 dilution + Human plasma lysate at 10 µg

      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

      Predicted band size: 53 kDa
      Observed band size: 52 kDa
      why is the actual band size different from the predicted?

      Exposure time: 3 minutes

      Blocking and Diluting buffer 5% NFDM /TBST 

    • Immunohistochemical analysis of paraffin-embedded human liver using ab81307 at a 1/100 dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.


    This product has been referenced in:

    • Petrov B  et al. Bipolar disorder in youth is associated with increased levels of vitamin D-binding protein. Transl Psychiatry 8:61 (2018). Read more (PubMed: 29531242) »
    • Li J  et al. Single-cell transcriptomes reveal characteristic features of human pancreatic islet cell types. EMBO Rep 17:178-87 (2016). Read more (PubMed: 26691212) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Western blot
    Mouse Tissue lysate - whole (Brain lysate, Liver lysate and human serum)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    30 µg
    Brain lysate, Liver lysate and human serum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Jan 28 2016

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