Product nameAnti-Vitamin D Binding protein antibody [EP2923Y]
See all Vitamin D Binding protein primary antibodies
DescriptionRabbit monoclonal [EP2923Y] to Vitamin D Binding protein
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human Vitamin D Binding protein aa 250-350. The exact sequence is proprietary.
- Human liver tissue. Human plasma.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab81307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMultifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
- Information by UniProt
- DBP antibody
- DBP/GC antibody
- GC antibody
Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307) at 1/20000 dilution + Human plasma lysate at 10 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking and Diluting buffer 5% NFDM /TBST
Immunohistochemical analysis of paraffin-embedded human liver using ab81307 at a 1/100 dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
This product has been referenced in:
- Petrov B et al. Bipolar disorder in youth is associated with increased levels of vitamin D-binding protein. Transl Psychiatry 8:61 (2018). Read more (PubMed: 29531242) »
- Li J et al. Single-cell transcriptomes reveal characteristic features of human pancreatic islet cell types. EMBO Rep 17:178-87 (2016). Read more (PubMed: 26691212) »