Recombinant Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)
![Western blot - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)](https://www.abcam.com/ps/products/81/ab81307/Images/ab81307-264172-anti-vitamin-d-binding-protein-antibody-ep2923y-western-blot.jpg "Western blot - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2923Y] to Vitamin D Binding protein
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Vitamin D Binding protein antibody [EP2923Y]
See all Vitamin D Binding protein primary antibodies -
Description
Rabbit monoclonal [EP2923Y] to Vitamin D Binding protein -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Vitamin D Binding protein aa 250-350. The exact sequence is proprietary.
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Positive control
- Human liver tissue. Human plasma.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2923Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/5000 - 1/20000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
|
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| Flow Cyt |
1/40.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/5000 - 1/20000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
|
Flow Cyt
1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2638 Human
- Omim: 139200 Human
- SwissProt: P02774 Human
- Unigene: 418497 Human
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Alternative names
- DBP antibody
- DBP/GC antibody
- GC antibody
see all
Images
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Western blot - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307) at 1/20000 dilution + Human plasma lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and Diluting buffer 5% NFDM /TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)](https://www.abcam.com/ps/products/81/ab81307/Images/ab81307-96520-anti-vitamin-d-binding-protein-antibody-ep2923y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)Immunohistochemical analysis of paraffin-embedded human liver using ab81307 at a 1/100 dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)](https://www.abcam.com/ps/products/81/ab81307/Images/ab81307-4-benefits-of-recombinant-antibodies.png)
Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab81307 has been referenced in 3 publications.
- Keeratichamroen S et al. Identification of potential cervical cancer serum biomarkers in Thai patients. Oncol Lett 19:3815-3826 (2020). PubMed: 32391095
- Petrov B et al. Bipolar disorder in youth is associated with increased levels of vitamin D-binding protein. Transl Psychiatry 8:61 (2018). PubMed: 29531242
- Li J et al. Single-cell transcriptomes reveal characteristic features of human pancreatic islet cell types. EMBO Rep 17:178-87 (2016). PubMed: 26691212