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Rabbit monoclonal [EPR2922Y] to Vitamin D Binding protein
ICC/IF more details Unsuitable for:
IHC-P or IP
Synthetic peptide within Human Vitamin D Binding protein aa 250-350. The exact sequence is proprietary.
Human Plasma and permeabilized U937 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium azide Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/20000. Predicted molecular weight: 53 kDa.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IHC-P or IP.
Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
Information by UniProt
Western blot - Anti-Vitamin D Binding protein antibody [EPR2922Y] (ab76537)
Anti-Vitamin D Binding protein antibody [EPR2922Y] (ab76537) at 1/20000 dilution + human plasma at 10 µg
Secondary HRP labelled goat anti-rabbit at 1/1000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa
Flow Cytometry - Anti-Vitamin D Binding protein antibody [EPR2922Y] (ab76537)
Flow cytometric analysis of permeabilized U937 cells using ab76537 at a 1/40 dilution (red) or a rabbit IgG (negative) (green).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"