Anti-Vitamin D Receptor antibody (ab134826)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Vitamin D Receptor antibody
See all Vitamin D Receptor primary antibodies -
Description
Rabbit polyclonal to Vitamin D Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISA, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to an internal sequence of Human Vitamin D Receptor.
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Positive control
- Human Skin tissue; HeLa cells; MCF7 cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 49% PBS, 50% Glycerol
PBS (without Mg2+, Ca2+) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 48 kDa. | |
| ELISA | 1/10000. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/500. |
Target
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Function
Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. -
Involvement in disease
Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7421 Human
- Omim: 601769 Human
- SwissProt: P11473 Human
- Unigene: 524368 Human
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Alternative names
- 1 25 dihydroxyvitamin D3 receptor antibody
- 1 antibody
- 1,25 dihydroxyvitamin D3 receptor antibody
see all
Images
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Western blot - Anti-Vitamin D Receptor antibody (ab134826)All lanes : Anti-Vitamin D Receptor antibody (ab134826) at 1/500 dilution
Lane 1 : MCF7 cell extracts
Lane 2 : MCF7 cell extracts with synthesized peptide
Predicted band size: 48 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Vitamin D Receptor antibody (ab134826)Immunofluorescence analysis of HeLa cells labelling Vitamin D Receptor with ab134826 at 1/100 dilution. The image on the right is treated with the synthesized peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (ab134826)Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human Skin tissue labelling Vitamin D Receptor with ab134826 at 5 µg/ml.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Sun W et al. 'Psoriasis 1' reduces psoriasis-like skin inflammation by inhibiting the VDR-mediated nuclear NF-?B and STAT signaling pathways. Mol Med Rep 18:2733-2743 (2018). Read more (PubMed: 30015892) »
