Anti-Vitamin D Receptor antibody (ab134826)
Key features and details
- Rabbit polyclonal to Vitamin D Receptor
- Suitable for: ELISA, WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Vitamin D Receptor antibody
See all Vitamin D Receptor primary antibodies -
Description
Rabbit polyclonal to Vitamin D Receptor -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to an internal sequence of Human Vitamin D Receptor.
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Positive control
- Human Skin tissue; HeLa cells; MCF7 cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 49% PBS, 50% Glycerol (glycerin, glycerine)
PBS (without Mg2+, Ca2+) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/1000. Predicted molecular weight: 48 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/500.
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| Notes |
|---|
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ELISA
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Predicted molecular weight: 48 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/500. |
Target
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Function
Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. -
Involvement in disease
Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7421 Human
- Omim: 601769 Human
- SwissProt: P11473 Human
- Unigene: 524368 Human
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Alternative names
- 1 25 dihydroxyvitamin D3 receptor antibody
- 1 antibody
- 1,25 dihydroxyvitamin D3 receptor antibody
see all
Images
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Western blot - Anti-Vitamin D Receptor antibody (ab134826)All lanes : Anti-Vitamin D Receptor antibody (ab134826) at 1/500 dilution
Lane 1 : MCF7 cell extracts
Lane 2 : MCF7 cell extracts with synthesized peptide
Predicted band size: 48 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Vitamin D Receptor antibody (ab134826)Immunofluorescence analysis of HeLa cells labelling Vitamin D Receptor with ab134826 at 1/100 dilution. The image on the right is treated with the synthesized peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (ab134826)Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human Skin tissue labelling Vitamin D Receptor with ab134826 at 5 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab134826 has been referenced in 4 publications.
- Docs J et al. Impaired Vitamin D Signaling Is Associated With Frequent Development of Renal Cell Tumor in End-stage Kidney Disease. Anticancer Res 40:6525-6530 (2020). PubMed: 33109593
- Ali M et al. Hypovitaminosis D exacerbates the DNA damage load in human uterine fibroids, which is ameliorated by vitamin D3 treatment. Acta Pharmacol Sin 40:957-970 (2019). PubMed: 30478352
- Ali M et al. 1,25 Dihydroxyvitamin D3 Enhances the Antifibroid Effects of Ulipristal Acetate in Human Uterine Fibroids. Reprod Sci 26:812-828 (2019). PubMed: 30514160
- Sun W et al. 'Psoriasis 1' reduces psoriasis-like skin inflammation by inhibiting the VDR-mediated nuclear NF-?B and STAT signaling pathways. Mol Med Rep 18:2733-2743 (2018). PubMed: 30015892
