Anti-Vitamin D Receptor antibody (ab3508)
Key features and details
- Rabbit polyclonal to Vitamin D Receptor
- Suitable for: IHC-Fr, ChIP, WB, IP, IHC-P, ChIP-sequencing, ICC/IF
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
Overview
-
Product name
Anti-Vitamin D Receptor antibody
See all Vitamin D Receptor primary antibodies -
Description
Rabbit polyclonal to Vitamin D Receptor -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, ChIP, WB, IP, IHC-P, ChIP-sequencing, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human
Predicted to work with: Cow, Pig, Zebrafish, Saguinus oedipus -
Immunogen
Synthetic peptide corresponding to Human Vitamin D Receptor aa 350-450.
Database link: P11473 -
Positive control
- IHC: Human jejunum
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
EMSA |
Use at an assay dependent concentration.
|
|
Gel Shift Assay |
Use at an assay dependent concentration.
|
|
IHC-Fr | (1) |
Use at an assay dependent concentration.
|
ChIP |
Use at an assay dependent concentration. PubMed: 17244627
|
|
WB | (3) |
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements). |
IP |
Use at an assay dependent concentration.
|
|
IHC-P | (1) |
1/2000 - 1/4000.
|
ChIP-sequencing |
Use at an assay dependent concentration. PubMed: 21846776
|
|
ICC/IF | (1) |
Use at an assay dependent concentration.
|
Notes |
---|
EMSA
Use at an assay dependent concentration. |
Gel Shift Assay
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. PubMed: 17244627 |
WB
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa. 1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements). |
IP
Use at an assay dependent concentration. |
IHC-P
1/2000 - 1/4000. |
ChIP-sequencing
Use at an assay dependent concentration. PubMed: 21846776 |
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. -
Involvement in disease
Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 7421 Human
- Entrez Gene: 22337 Mouse
- Entrez Gene: 24873 Rat
- Omim: 601769 Human
- SwissProt: P11473 Human
- SwissProt: P48281 Mouse
- SwissProt: P13053 Rat
- Unigene: 524368 Human
see all -
Alternative names
- 1 25 dihydroxyvitamin D3 receptor antibody
- 1 antibody
- 1,25 dihydroxyvitamin D3 receptor antibody
see all
Images
-
Ab3508 staining Human normal jejunum. Staining is localized to the nucleus.
Left panel: with primary antibody at 1/2000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (66)
ab3508 has been referenced in 66 publications.
- Khazan N et al. Identification of a Vitamin-D Receptor Antagonist, MeTC7, which Inhibits the Growth of Xenograft and Transgenic Tumors In Vivo. J Med Chem 65:6039-6055 (2022). PubMed: 35404047
- Zhang WH et al. Fecal Microbiota Transplantation Ameliorates Active Ulcerative Colitis by Downregulating Pro-inflammatory Cytokines in Mucosa and Serum. Front Microbiol 13:818111 (2022). PubMed: 35444617
- Heidegger I et al. Comprehensive characterization of the prostate tumor microenvironment identifies CXCR4/CXCL12 crosstalk as a novel antiangiogenic therapeutic target in prostate cancer. Mol Cancer 21:132 (2022). PubMed: 35717322
- Nizami HL et al. Paricalcitol Attenuates Metabolic Syndrome-Associated Heart Failure through Enhanced Mitochondrial Fusion. Oxid Med Cell Longev 2022:5554290 (2022). PubMed: 35726330
- Guan H et al. DNA methyltransferase 1 knockdown reverses PTEN and VDR by mediating demethylation of promoter and protects against renal injuries in hepatitis B virus-associated glomerulonephritis. Cell Biosci 12:98 (2022). PubMed: 35765066