Anti-Vitamin D Receptor antibody - ChIP Grade (ab3508)

Rabbit polyclonal Vitamin D Receptor antibody. Validated in WB, IP, IHC, ChIP, ICC/IF, CHIPseq and tested in Mouse, Rat, Chicken, Human. Cited in 28 publication(s). Independently reviewed in 3 review(s).


  • Product name

    Anti-Vitamin D Receptor antibody - ChIP Grade
    See all Vitamin D Receptor primary antibodies
  • Description

    Rabbit polyclonal to Vitamin D Receptor - ChIP Grade
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, IP, WB, CHIPseq, IHC-P, ChIPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human
    Predicted to work with: Cow, Pig, Zebrafish, Saguinus oedipus
  • Immunogen

    Synthetic peptide corresponding to Human Vitamin D Receptor aa 395-413.


    Database link: P11473



Our Abpromise guarantee covers the use of ab3508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/100. Detects a band of approximately 53 kDa (predicted molecular weight: 48 kDa). 1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements).
CHIPseq Use at an assay dependent concentration. PubMed: 21846776
IHC-P 1/2000 - 1/4000.
ChIP Use at an assay dependent concentration. PubMed: 17244627Use at an assay dependent dilution.


  • Function

    Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
  • Involvement in disease

    Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • 1 25 dihydroxyvitamin D3 receptor antibody
    • 1 antibody
    • 1,25 dihydroxyvitamin D3 receptor antibody
    • 1,25-@dihydroxyvitamin D3 receptor antibody
    • 25-dihydroxyvitamin D3 receptor antibody
    • Member 1 antibody
    • NR1I1 antibody
    • Nuclear receptor subfamily 1 group I member 1 antibody
    • PPP1R163 antibody
    • Protein phosphatase 1, regulatory subunit 163 antibody
    • VDR antibody
    • VDR_HUMAN antibody
    • Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody
    • Vitamin D hormone receptor antibody
    • Vitamin D nuclear receptor variant 1 antibody
    • Vitamin D receptor antibody
    • Vitamin D3 receptor antibody
    see all


  • Ab3508 staining Human normal jejunum. Staining is localized to the nucleus.
    Left panel: with primary antibody at 1/2000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • ab3508 staining the Vitamin D Receptor in mVDR-transfected and untransfected Mouse NIH/3T3 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with 1% BSA/5%HS for 30 minutes at 20°C. The sample was incubated with the primary antibody (1/50 in 1% BSA/5% HS in 1xPBS) for 1 hour 30 minutes at 20°C. A Cy3®-conjugated goat anti-rabbit polyclonal (1/200) was used as the secondary.

    See Abreview

  • ab3508 at a 1/2000 dilution staining Vitamin D Receptor in whole Rat embryo tissue sections by Immunohistochemistry (frozen sections) incubated for 16 hours at 25°C. Samples fixed in 4% PFA prior to cutting at 30µm thickness. Blocked with 5% serum for 1 hour at 25°C. Secondary used at 1/200 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488.

    See Abreview


This product has been referenced in:

  • Qin T  et al. Prediction of the mechanisms of action of Shenkang in chronic kidney disease: A network pharmacology study and experimental validation. J Ethnopharmacol 246:112128 (2020). Read more (PubMed: 31386888) »
  • Lee JS  et al. Claudin-1 expression decreases with increasing pathological grade in actinic keratosis and may be a marker of high-risk actinic keratosis. Clin Exp Dermatol 44:483-490 (2019). Read more (PubMed: 30315595) »
See all 32 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Mouse Cell (NIH/3T3, untransfected, mVDR-transfected)
NIH/3T3, untransfected, mVDR-transfected
Yes - Triton X-100
Blocking step
1% BSA, 5% HS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Miss. A Uhmann

Verified customer

Submitted Dec 03 2009


Thank you for contacting us.

Since this is a whole antiserum antibody, the concentration has not been determined. Typically the total protein concentration for these antibodies can range from 1 to 10 mg/ml (you could split the difference and estimate 5 mg/ml).

You may want to remove the additional protein blocking step after the primary antibody incubation. Plus he antigen retrieval step may be a bit too long. Typically 10 mins boiling in a microwave followed by 20 min cooling works well. Also, since this is a nuclear antigen, you should permeabilize with Triton X-100 if you're not already. What species are your samples from and what is the tissue type (i.e. rat spleen)?

I'm listing some references below which have cited the use of this antibody in IHC:

Arch Biochem Biophys. 2010 Feb 15;494(2):166-77.
"Identification of a highly specific and versatile vitamin D receptor antibody."
Author(s): Wang Y, Becklund BR, DeLuca HF
Number of Citations: 1

Biogerontology. 2008 Apr;9(2):109-18.
"Vitamin D receptor levels and binding are reduced in aged rat intestinal subcellular fractions."
Author(s): González Pardo V, Boland R, de Boland AR
Number of Citations: 1

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link:

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Thank you very much for contacting us with your enquiry.

Ab3508 is an unpurified whole serum antibody, and as there are other proteins in the serum the concentration of specific IgG can not be measured. This antibody has been used in ChIP in 4 known publications, though it's not tested in ChIP in our own lab. I've looked through the references (attached) and in "Ab3508 ChIP 1" and the protocol references of "Ab3508 ChIP 2, the authors used 5 uL of ab3508 per chromatin pellet.

I am sorry that we don't have more information, but I hope this will be useful. If you have any further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

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Immunohistochemistry (Frozen sections)
Rat Tissue sections (Whole embryo)
Whole embryo
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 14 2009

Western blot
Mouse Cell lysate - nuclear (NIH/3T3, untransfected, VDR-transfected)
Loading amount
10 µg
NIH/3T3, untransfected, VDR-transfected
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
I-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C

Miss. A Uhmann

Verified customer

Submitted Dec 01 2009


Thank you for your enquiry. The resources I have available indicate that 1 ul of ab3508 should be a sufficient amount for immunoprecipitation assays. I would recommend using a minimum of 100 ug of sample. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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