Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Vitamin D Receptor antibody [EPR4552] - BSA and Azide free (ab239958)

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Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - BSA and Azide free (ab239958)
  • ChIP - Anti-Vitamin D Receptor antibody [EPR4552] - BSA and Azide free (ab239958)
  • Immunoprecipitation - Anti-Vitamin D Receptor antibody [EPR4552] - BSA and Azide free (ab239958)
  • Anti-Vitamin D Receptor antibody [EPR4552] - BSA and Azide free (ab239958)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4552] to Vitamin D Receptor - BSA and Azide free
  • Suitable for: ChIP, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Vitamin D Receptor antibody [EPR4552] - BSA and Azide free
    See all Vitamin D Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR4552] to Vitamin D Receptor - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ChIP, IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Vitamin D Receptor aa 1-100. The exact sequence is proprietary.
    Database link: P11473
    (Peptide available as ab198626)

  • General notes

    Ab239958 is the carrier-free version of ab109234. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239958 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab239958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.

    • Involvement in disease

      Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.

    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.

    • Domain

      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 1 25 dihydroxyvitamin D3 receptor antibody
      • 1 antibody
      • 1,25 dihydroxyvitamin D3 receptor antibody
      • 1,25-@dihydroxyvitamin D3 receptor antibody
      • 25-dihydroxyvitamin D3 receptor antibody
      • Member 1 antibody
      • NR1I1 antibody
      • Nuclear receptor subfamily 1 group I member 1 antibody
      • PPP1R163 antibody
      • Protein phosphatase 1, regulatory subunit 163 antibody
      • VDR antibody
      • VDR_HUMAN antibody
      • Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody
      • Vitamin D hormone receptor antibody
      • Vitamin D nuclear receptor variant 1 antibody
      • Vitamin D receptor antibody
      • Vitamin D3 receptor antibody
      see all

    Images

    • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - BSA and Azide free (ab239958)
      Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - BSA and Azide free (ab239958)
      All lanes : Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/1000 dilution

      Lane 1 : Wild-type HeLa lysate
      Lane 2 : Vitamin D Receptor knockout HeLa lysate
      Lane 3 : U-937 lysate
      Lane 4 : SH-SY5Y lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 48 kDa



      This data was developed using the same antibody clone in a different buffer formulation (ab109234).

      Lanes 1-4: Merged signal (red and green). Green - ab109234 observed at 50 kDa. Red - loading control ab8245 observed at 37 kDa.

       ab109234 Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade was shown to specifically react with Vitamin D Receptor in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265430 (knockout cell lysate ab257796) was used. Wild-type and Vitamin D Receptor knockout samples were subjected to SDS-PAGE. ab109234 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

       

    • ChIP - Anti-Vitamin D Receptor antibody [EPR4552] - BSA and Azide free (ab239958)
      ChIP - Anti-Vitamin D Receptor antibody [EPR4552] - BSA and Azide free (ab239958)

      Chromatin was prepared from T-47D cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25 µg of chromatin, 2 µg of ab223850 (blue), and 20 µL of protein A/G sepharose beads slurry (10 µL of sepharose A beads + 10 µL of sepharose G beads). 5 μg of rabbit normal IgG was added to the beads as a control sample (yellow). The immunoprecipitated DNA was quantified by real time PCR (SYBR Green chemistry) with primers to c-Fos.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109234).

    • Immunoprecipitation - Anti-Vitamin D Receptor antibody [EPR4552] - BSA and Azide free (ab239958)
      Immunoprecipitation - Anti-Vitamin D Receptor antibody [EPR4552] - BSA and Azide free (ab239958)

      Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate, 10 μg
      Lane 2(+): T-47D whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109234 in T-47D whole cell lysate

      Ab109234 immunoprecipitating Vitamin D receptor in T-47D whole cell lysates. Capture antibody was used at a 1/30 dilution (2 μg in 0.35 mg lysates). For western blotting, primary antibody was used as ab109234 at 1/1000 dilution (0.62 μg/mL). VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109234).

    • Anti-Vitamin D Receptor antibody [EPR4552] - BSA and Azide free (ab239958)
      Anti-Vitamin D Receptor antibody [EPR4552] - BSA and Azide free (ab239958)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab239958? Please let us know so that we can cite the reference in this datasheet.

    ab239958 has not yet been referenced specifically in any publications.

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