Key features and details
- Rabbit polyclonal to Vitamin D Receptor (phospho S208)
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Vitamin D Receptor (phospho S208) antibody
See all Vitamin D Receptor primary antibodies
DescriptionRabbit polyclonal to Vitamin D Receptor (phospho S208)
SpecificityDetects Vitamin D Receptor only when phosphorylated at serine 208.
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic phosphopeptide derived from human Vitamin D Receptor around the phosphorylation site of serine 208 (D-L-SP-E-E).
- A549 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Vitamin D Receptor
Our Abpromise guarantee covers the use of ab63572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
|ICC/IF||1/500 - 1/1000.|
FunctionNuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
Involvement in diseaseDefects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
- Information by UniProt
- 1 25 dihydroxyvitamin D3 receptor antibody
- 1 antibody
- 1,25 dihydroxyvitamin D3 receptor antibody
Immunofluorescent staining of A549 cells, using ab63572 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).
Serum starved U937 cells were incubated with 0.5 µM H2O2, 10 µg/ml A24, or 3 µM DFO for 10 min at 37°C. Phosphorylation of VDR (P-VDR) in whole cell extracts was analyzed using ab63572.
ab63572 has been referenced in 1 publication.
- Callens C et al. Targeting iron homeostasis induces cellular differentiation and synergizes with differentiating agents in acute myeloid leukemia. J Exp Med 207:731-50 (2010). WB ; Human . PubMed: 20368581