Recombinant
RabMAb

Recombinant Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)

Overview

  • Product name

    Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12]
    See all Vitamin D2+Vitamin D3 primary antibodies
  • Description

    Rabbit monoclonal [EPPTX-6-271-12] to Vitamin D2+Vitamin D3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Vitamin D2+Vitamin D3.

  • Positive control

    • Competitive ELISA: 25-OH-Vitamin-D.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPPTX-6-271-12
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Use at 50-60 ng/ml.

Target

  • Cellular localization

    Vitamin D2: Secreted Vitamin D3: Secreted

Images

  • Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 50-60 ng/mL of ab229822 (50 μL) for 30 minutes by shaking.
    This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

  • ab229822 was used at 11.8 ng/ml.

    The results show cross-reactivity with Vitamin D2.
    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

References

ab229822 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229822.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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