Recombinant Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)
![Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)](https://www.abcam.com/ps/products/229/ab229822/Images/ab229822-1-229822ELISA1.jpg "Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-6-271-12] to Vitamin D2+Vitamin D3
- Suitable for: Competitive ELISA
- Reacts with: Species independent
Overview
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Product name
Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12]
See all Vitamin D2+Vitamin D3 primary antibodies -
Description
Rabbit monoclonal [EPPTX-6-271-12] to Vitamin D2+Vitamin D3 -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Vitamin D2+Vitamin D3.
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Positive control
- Competitive ELISA: 25-OH-Vitamin-D.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTX-6-271-12 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab229822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Competitive ELISA | Use at an assay dependent concentration. Use at 50-60 ng/ml. |
Target
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Cellular localization
Vitamin D2: Secreted Vitamin D3: Secreted
Images
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Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 50-60 ng/mL of ab229822 (50 μL) for 30 minutes by shaking.
This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)](https://www.abcam.com/ps/products/229/ab229822/Images/ab229822-2-229822ELISA2.jpg)
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)ab229822 was used at 11.8 ng/ml.
The results show cross-reactivity with Vitamin D2.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc. -
![Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)](https://www.abcam.com/ps/products/229/ab229822/Images/ab229822-3-benefits-of-recombinant-antibodies.png)
Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (ab229822)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab229822 has not yet been referenced specifically in any publications.
