Recombinant Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (ab229821)
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Overview
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Product name
Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3]
See all Vitamin D2+Vitamin D3 primary antibodies -
Description
Rabbit monoclonal [EPPTX-R6-74-3] to Vitamin D2+Vitamin D3 -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule. Vitamin-D-3-BSA.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTX-R6-74-3 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab229821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Competitive ELISA | Use at an assay dependent concentration. Use at 30-35 ng/mL. |
Target
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Cellular localization
Vitamin D2: Secreted Vitamin D3: Secreted
Images
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![Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (ab229821)](https://www.abcam.com/ps/products/229/ab229821/Images/ab229821-1-229821ELISA1.jpg)
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (ab229821)Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (ab229821).
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 30-35 ng/mL of ab229821 (50 μL) for 30 minutes by shaking.
This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (ab229821)](https://www.abcam.com/ps/products/229/ab229821/Images/ab229821-2-229821ELISA2.jpg)
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (ab229821)Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (ab229821) used at 8.3 ng/mL.
The results show cross-reactivity with Vitamin D2.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
Datasheets and documents
References
ab229821 has not yet been referenced specifically in any publications.
