Recombinant Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)
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Overview
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Product name
Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free
See all Vitamin D2+Vitamin D3 primary antibodies -
Description
Rabbit monoclonal [EPPTX-R6-74-3] to Vitamin D2+Vitamin D3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Vitamin D2+Vitamin D3. Vitamin-D-3-BSA.
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General notes
Ab236471 is the carrier-free version of ab229821. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab236471 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTX-R6-74-3 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab236471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Competitive ELISA | Use at an assay dependent concentration. Use at 30-35 ng/mL. |
Target
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Cellular localization
Vitamin D2: Secreted Vitamin D3: Secreted
Images
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![Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)](https://www.abcam.com/ps/products/236/ab236471/Images/ab236471-312600-236471.jpg)
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (ab229821).
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 30-35 ng/mL of ab229821 (50 μL) for 30 minutes by shaking.
This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)](https://www.abcam.com/ps/products/236/ab236471/Images/ab236471-312599-2364712.jpg)
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (ab236471)Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (ab229821) used at 8.3 ng/mL.
The results show cross-reactivity with Vitamin D2.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
Datasheets and documents
References
ab236471 has not yet been referenced specifically in any publications.
