Recombinant
RabMAb

Recombinant Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)

Overview

  • Product name

    Anti-Vitamin D3 antibody [EPPTX-R6-42-12]
    See all Vitamin D3 primary antibodies
  • Description

    Rabbit monoclonal [EPPTX-R6-42-12] to Vitamin D3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Chemical/ Small Molecule. Vitamin-D-3-BSA.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPPTX-R6-42-12
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Use at 30-35 ng/ml.

Target

  • Relevance

    Vitamin D3, also known as cholecalciferol, is a form of vitamin D that is made by the human body. It is made in the skin when 7-dehydrocholesterol reacts with UVB ultraviolet light with wavelengths 290 to 315 nm. This can be found in sunlight when the sun is high enough above the horizon for UVB to penetrate the atmosphere and is responsible for the production of cholecalciferol. Up to 20,000 IU can be made in the skin only after one minimal erythemal dose of exposure, or until the skin just begins to turn pink.
  • Cellular localization

    Secreted
  • Alternative names

    • cholecalciferol antibody

Images

  • Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)

    Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 20-25 ng/mL of ab229820 (50 μL) for 30 minutes by shaking.

    This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

  • Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820) used at 6.3 ng/ml.

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

References

ab229820 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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