Recombinant
RabMAb

Recombinant Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free (ab236470)

Overview

  • Product name

    Anti-Vitamin D3 antibody [EPPTX-R6-42-12] - BSA and Azide free
    See all Vitamin D3 primary antibodies
  • Description

    Rabbit monoclonal [EPPTX-R6-42-12] to Vitamin D3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Chemical/ Small Molecule corresponding to Vitamin D3. Vitamin-D-3-BSA.

  • General notes

    Ab236470 is the carrier-free version of ab229820. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236470 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPPTX-R6-42-12
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Use at 30-35 ng/mL.

Target

  • Relevance

    Vitamin D3, also known as cholecalciferol, is a form of vitamin D that is made by the human body. It is made in the skin when 7-dehydrocholesterol reacts with UVB ultraviolet light with wavelengths 290 to 315 nm. This can be found in sunlight when the sun is high enough above the horizon for UVB to penetrate the atmosphere and is responsible for the production of cholecalciferol. Up to 20,000 IU can be made in the skin only after one minimal erythemal dose of exposure, or until the skin just begins to turn pink.
  • Cellular localization

    Secreted
  • Alternative names

    • cholecalciferol antibody

Images

  • Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)

    Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 20-25 ng/mL of ab229820 (50 μL) for 30 minutes by shaking.

    This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

  • Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820) used at 6.3 ng/ml.

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

References

ab236470 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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