Overview

  • Product name
    Anti-Vitellogenin antibody
    See all Vitellogenin primary antibodies
  • Description
    Rabbit polyclonal to Vitellogenin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with Arctic char. It cross reacts with Atlantic salmon (Salmo salar), brown trout (Salmo trutta), Atlantic cod (Gadus morhua), turbot (Scophthalmus maximus), flounder (Platichthys flesus), wrasse (Ctenolabrus rupestris) and tilapia (Oreochromis niloticus), and zebrafish (Danio rerio). Not yet tested in other species.
  • Immunogen

    Vitellogenin purified from plasma of 17 beta estradiol treated Arctic char.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1:1000 - 1:3000 (Salmonids)
    1:500 - 1:1000 (Other species)

    WB: 1:1000 - 1:3000 (Salmonids)
    1:500 - 1:1000 (Other species) . Predicted molecular weight: 183 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
    • Cellular localization
      Secreted
    • Alternative names
      • Vg antibody
      • VTG antibody
      • VTG1 antibody

    Images

    • Coating: Plasma (diluted 1:1000) from 17 beta-estradiol treated (E2), sexually mature female (E) or control (C) fish. Primary antibody: PO-1
    • All lanes : Anti-Vitellogenin antibody (ab36881) at 1/3000 dilution

      Lane 1 : MW standard
      Lane 2 : Salmon (E2)
      Lane 3 : Salmon (C)
      Lane 4 : Cod (E2)
      Lane 5 : Cod (C)
      Lane 6 : Carp (E2)
      Lane 7 : Carp (C)

      Predicted band size: 183 kDa



      Samples: Plasma (1:200, 10 µl/well) from 17 beta-estradiol treated (E2) or control (C) fish.

    References

    ab36881 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    For our records, could you please send us the name and the email address of your customer? DISCOUNT CODE: ******** Expiration date: DD MM YYYY I am very pleased to hear your customer would like to accept our offer and test ab36881 in Bemisia tabaci whitefly. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for Bemisia tabaci whitefly and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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