Overview

  • Product name

    Anti-Vitellogenin antibody [BN-5]
    See all Vitellogenin primary antibodies
  • Description

    Mouse monoclonal [BN-5] to Vitellogenin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Rainbow trout, Salmo salar
  • Immunogen

    Full length native vitellogenin purified from plasma of 17 beta estradiol induced Atlantic salmon (Salmo salar).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    BN-5
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 180 kDa.
ELISA 1/100 - 1/2000.

Target

  • Relevance

    Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
  • Cellular localization

    Secreted
  • Database links

    • Alternative names

      • Vg antibody
      • VTG antibody
      • VTG1 antibody

    Images

    • Coating: Plasma (diluted 1:1000) from 17 beta-estradiol treated (E2), sexually mature female (E) or control (C) fish. Primary antibody: BN-5.
    • All lanes : Anti-Vitellogenin antibody [BN-5] (ab36797) at 1/100 dilution

      Lane 1 : MW Standard
      Lane 2 : Salmon (C)
      Lane 3 : Salmon (E2)
      Lane 4 : Brown trout (C)
      Lane 5 : Brown trout (E)
      Lane 6 : Rainbow trout (C)
      Lane 7 : Rainbow trout (E2)

      Predicted band size: 180 kDa



      Samples: Plasma (1:200, 7 µl/well) from 17 beta-estradiol treated (E2), sexually mature female (E) or control (C) fish.

    References

    This product has been referenced in:

    • Zheng R  et al. Joint effects of chronic exposure to environmentally relevant levels of nonylphenol and cadmium on the reproductive functions in male rockfish Sebastiscus marmoratus. Comp Biochem Physiol C Toxicol Pharmacol 215:25-32 (2019). Read more (PubMed: 30315922) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Fish Tissue sections (gonads of Salmo trutta m. fario & Salmo trutta m.)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric buffer pH6
    Permeabilization
    No
    Specification
    gonads of Salmo trutta m. fario & Salmo trutta m.
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Bouin's solution

    Dr. Dobrochna Adamek

    Verified customer

    Submitted Jul 19 2019

    Answer

    Thank you for contacting us and your interest in our products.
    We do have an antibody which has been raised against full length native vitellogenin purified from plasma of 17 beta estradiol induced Atlantic Salmon (Salmo salar) which may be suitable for your needs. This antibody is a mouse monoclonal, ab36797 which has been validated in western blotting and ELISA.
    I hope this information has been of help. If you require any further information please do not hesitate to let us know.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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