Key features and details
- Mouse monoclonal [BN-5] to Vitellogenin
- Suitable for: WB, ELISA
- Reacts with: Rainbow trout, Salmo salar
- Isotype: IgG1
Product nameAnti-Vitellogenin antibody [BN-5]
See all Vitellogenin primary antibodies
DescriptionMouse monoclonal [BN-5] to Vitellogenin
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Rainbow trout, Salmo salar
Full length native vitellogenin purified from plasma of 17 beta estradiol induced Atlantic salmon (Salmo salar).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab36797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 180 kDa.|
|ELISA||1/100 - 1/2000.|
RelevanceVitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
- SwissProt: Q92093 Rainbow trout
- Vg antibody
- VTG antibody
- VTG1 antibody
Coating: Plasma (diluted 1:1000) from 17 beta-estradiol treated (E2), sexually mature female (E) or control (C) fish. Primary antibody: BN-5.
All lanes : Anti-Vitellogenin antibody [BN-5] (ab36797) at 1/100 dilution
Lane 1 : MW Standard
Lane 2 : Salmon (C)
Lane 3 : Salmon (E2)
Lane 4 : Brown trout (C)
Lane 5 : Brown trout (E)
Lane 6 : Rainbow trout (C)
Lane 7 : Rainbow trout (E2)
Predicted band size: 180 kDa
Samples: Plasma (1:200, 7 µl/well) from 17 beta-estradiol treated (E2), sexually mature female (E) or control (C) fish.
ab36797 has been referenced in 1 publication.
- Zheng R et al. Joint effects of chronic exposure to environmentally relevant levels of nonylphenol and cadmium on the reproductive functions in male rockfish Sebastiscus marmoratus. Comp Biochem Physiol C Toxicol Pharmacol 215:25-32 (2019). PubMed: 30315922